ELISA/assay

Human Kallikrein 5 ELISA Kit

MBS824564

96 Tests

515 USD

ELISA Kit

Human Kallikrein 5 ELISA Kit

[Kallikrein 5]

[SCTE; Kallikrein-5; Kallikrein-like protein 2; KLK-L2; Stratum corneum tryptic enzyme]

[kallikrein-5 preproprotein; Kallikrein-5; kallikrein-5; kallikrein 5; kallikrein-like protein 2; stratum corneum tryptic enzyme; kallikrein-related peptidase 5; Kallikrein-like protein 2; KLK-L2; Stratum corneum tryptic enzyme]

[KLK5]

[KLK5; KLK5; SCTE; KLKL2; KLK-L2; SCTE; KLK-L2]

KLK5_HUMAN

Human

293

The Human Kallikrein 5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Kallikrein 5 proteins within the range of 78 pg/ml - 5000 pg/ml.

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell Culture Supernatants, Serum, Plasma. Sensitivity: 10 pg/ml

Principle of the Assay: The Human Kallikrein 5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Kallikrein 5 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Kallikrein 5 coated on a 96-well plate. Standards and samples are pipetted into the wells and Kallikrein 5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Kallikrein 5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Kallikrein 5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14. -FOR

117306172

NP_001070959.1

NM_001077491.1

Q9Y337

32,019 Da

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

KLK5: May be involved in desquamation. Belongs to the peptidase S1 family. Kallikrein subfamily. Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.-; Protease. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: extracellular space. Molecular Function: peptidase activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: epidermis development; positive regulation of G-protein coupled receptor protein signaling pathway; proteolysis

96 Tests

515 USD