Anti-Axin-2 Antibody | MyBioSource MBS8245445 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Axin-2 Antibody

catalog :

MBS8245445

quantity :

0.05 mL

price :

175 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Axin-2 expression in mouse lung (A), mouse brain (B), mouse liver (C) whole cell lysates.

image 2 :

Immunofluorescent analysis of Axin-2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8245445

products type :

Antibody

products full name :

Anti-Axin-2 Antibody

products short name :

[Axin-2]

products name syn :

[Axin-2; Axin-like protein; Axil; Axis inhibition protein 2; Conductin]

other names :

[axin-2; Axin-2; axin-2; axin 2; Axin-like protein; Axil; Axis inhibition protein 2; Conductin]

products gene name :

[AXIN2]

other gene names :

[AXIN2; AXIN2; AXIL; ODCRCS; Axil]

uniprot entry name :

AXIN2_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

843

specificity :

Recognizes endogenous levels of Axin-2 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: Recombinant full length protein of human Axin-2

products description :

Rabbit polyclonal antibody to Axin-2

ncbi gi num :

195927059

ncbi acc num :

NP_004646.3

ncbi gb acc num :

NM_004655.3

uniprot acc num :

Q9Y2T1

ncbi mol weight :

93,558 Da

ncbi pathways :

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Binding Of TCF/LEF:CTNNB1 To Target Gene Promoters Pathway (1269603); Canonical Wnt Signaling Pathway (138032); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518); Degradation Of AXIN Pathway (1269606); Endometrial Cancer Pathway (83109); Endometrial Cancer Pathway (521); Formation Of The Beta-catenin:TCF Transactivating Complex Pathway (1269602)

ncbi summary :

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

uniprot summary :

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17q24.1. Cellular Component: beta-catenin destruction complex; cell cortex; centrosome; cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasmic microtubule; cytosol; Golgi apparatus; microtubule cytoskeleton; nucleoplasm; nucleus; plasma membrane; postsynaptic density. Molecular Function: beta-catenin binding; enzyme binding; GTPase activator activity; protein binding; protein kinase binding; ubiquitin protein ligase binding. Biological Process: bone mineralization; cell proliferation; dorsal/ventral axis specification; intramembranous ossification; maintenance of DNA repeat elements; mRNA stabilization; negative regulation of cell proliferation; negative regulation of osteoblast differentiation; odontogenesis; positive regulation of GTPase activity; positive regulation of protein amino acid phosphorylation; regulation of mismatch repair; regulation of transcription, DNA-dependent; Wnt receptor signaling pathway. Disease: Oligodontia-colorectal Cancer Syndrome

size1 :

0.05 mL

price1 :

175 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage