product summary
Loading...
company name :
MyBioSource
product type :
antibody
product name :
Anti-Sox-5 Antibody
catalog :
MBS8245243
quantity :
0.05 mL
price :
175 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of Sox-5 expression in HepG2 (A), SHSY5Y (B), mouse heart (C) whole cell lysates.
image 2 :
Immunohistochemical analysis of Sox-5 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
image 3 :
Immunofluorescent analysis of Sox-5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
product information
catalog number :
MBS8245243
products type :
Antibody
products full name :
Anti-Sox-5 Antibody
products short name :
[Sox-5]
products name syn :
[Transcription factor SOX-5]
other names :
[transcription factor SOX-5 isoform d; Transcription factor SOX-5; transcription factor SOX-5; SRY-box 5]
products gene name :
[SOX5]
other gene names :
[SOX5; SOX5; L-SOX5; LAMSHF; L-SOX5B; L-SOX5F]
uniprot entry name :
SOX5_HUMAN
clonality :
Polyclonal
host :
Rabbit
reactivity :
Human, Mouse, Rat
specificity :
Recognizes endogenous levels of Sox-5 protein.
purity :
The antibody was purified by immunogen affinity chromatography.
form :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
storage stability :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
tested application :
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
app notes :
WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/100
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Immunofluorescence (IF)
other info1 :
Immunogen: Recombinant full length protein of human Sox-5
products description :
Rabbit polyclonal antibody to Sox-5
ncbi gi num :
387157920
ncbi acc num :
NP_001248343.1
ncbi gb acc num :
NM_001261414.1
uniprot acc num :
P35711
ncbi pathways :
Endochondral Ossification Pathway (198812); Neural Crest Differentiation Pathway (672460)
ncbi summary :
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
SOX5: Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro. 4 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: nucleus. Molecular Function: DNA binding; protein binding; transcription factor activity. Biological Process: asymmetric neuroblast division; positive regulation of chondrocyte differentiation; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
size1 :
0.05 mL
price1 :
175 USD
size2 :
0.1 mL
price2 :
220
size3 :
0.2 mL
price3 :
295
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!
browse more products
questions and comments