Anti-AGAT Antibody | MyBioSource MBS8245218 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-AGAT Antibody

catalog :

MBS8245218

quantity :

0.05 mL

price :

175 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of AGAT expression in mouse kidney (A), rat kidney (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of AGAT staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of AGAT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8245218

products type :

Antibody

products full name :

Anti-AGAT Antibody

products short name :

[AGAT]

products name syn :

[AGAT; Glycine amidinotransferase, mitochondrial; L-arginine:glycine amidinotransferase; Transamidinase]

other names :

[glycine amidinotransferase, mitochondrial isoform 1; Glycine amidinotransferase, mitochondrial; glycine amidinotransferase, mitochondrial; glycine amidinotransferase; L-arginine:glycine amidinotransferase; Transamidinase]

products gene name :

[GATM]

other gene names :

[GATM; GATM; AT; AGAT; CCDS3; AGAT]

uniprot entry name :

GATM_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

423

specificity :

Recognizes endogenous levels of AGAT protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: Recombinant full length protein of human AGAT

products description :

Rabbit polyclonal antibody to AGAT

ncbi gi num :

4503933

ncbi acc num :

NP_001473.1

ncbi gb acc num :

NM_001482.2

uniprot acc num :

P50440

ncbi mol weight :

44,943 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (1270169); Creatine Pathway (413359); Creatine Pathway (468234); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Amino Acids And Derivatives Pathway (1270158)

ncbi summary :

This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders. [provided by RefSeq, Jul 2008]

uniprot summary :

GATM: Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development. May be involved in the response to heart failure by elevating local creatine synthesis. Defects in GATM are the cause of arginine:glycine amidinotransferase deficiency (AGAT deficiency). AGAT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, and severe depletion of creatine/phosphocreatine in the brain. Belongs to the amidinotransferase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transferase; Amino Acid Metabolism - arginine and proline; Mitochondrial; EC 2.1.4.1; Amino Acid Metabolism - glycine, serine and threonine. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: mitochondrial inner membrane; mitochondrial intermembrane space. Molecular Function: glycine amidinotransferase activity. Biological Process: creatine biosynthetic process; creatine metabolic process. Disease: Cerebral Creatine Deficiency Syndrome 3

size1 :

0.05 mL

price1 :

175 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage