ELISA/assay

Human CD36 ELISA Kit

MBS824511

96 Tests

515 USD

ELISA Kit

Human CD36 ELISA Kit

[CD36]

[GP3B; GP4; Platelet glycoprotein 4; Fatty acid translocase; FAT; Glycoprotein IIIb; GPIIIB; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; Platelet glycoprotein IV; GPIV; Thrombospondin receptor; CD36]

[platelet glycoprotein 4 isoform 1; Platelet glycoprotein 4; platelet glycoprotein 4; GPIIIB; PAS IV; PAS-4 protein; glycoprotein IIIb; cluster determinant 36; fatty acid translocase; platelet glycoprotein IV; scavenger receptor class B, member 3; leukocyte differentiation antigen CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); Fatty acid translocase; FAT; Glycoprotein IIIb; GPIIIB; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; Platelet glycoprotein IV; GPIV; Thrombospondin receptor; CD_antigen: CD36]

[CD36]

[CD36; CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10; GP3B; GP4; FAT; GPIIIB; GPIV]

CD36_HUMAN

Human

472

The Human CD36 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD36 proteins within the range of 156 pg/ml - 10000 pg/ml.

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell Culture Supernatants, Serum, Plasma. Sensitivity: 10 pg/ml.

Principle of the Assay: The Human CD36 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD36 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD36 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD36 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD36 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD36 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background: CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.

48375178

NP_000063.2

NM_000072.3

P16671

46,090 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activated TLR4 Signalling Pathway (106400); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Antigen Processing-Cross Presentation Pathway (477122); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway (477123)

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]

CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family. Protein type: Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 7q11.2. Cellular Component: Golgi apparatus; extracellular space; platelet alpha granule membrane; cell surface; membrane; integral to plasma membrane; plasma membrane; phagocytic vesicle; external side of plasma membrane; lipid raft. Molecular Function: low-density lipoprotein receptor activity; transforming growth factor beta binding; low-density lipoprotein binding; high-density lipoprotein binding; lipid binding. Biological Process: positive regulation of blood coagulation; positive regulation of interleukin-12 production; cGMP-mediated signaling; phagocytosis, recognition; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; negative regulation of transcription factor import into nucleus; low density lipoprotein mediated signaling; sequestering of lipid; antigen processing and presentation of peptide antigen via MHC class I; cell surface receptor linked signal transduction; platelet degranulation; positive regulation of MAPKKK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell adhesion; positive regulation of cell-matrix adhesion; toll-like receptor 4 signaling pathway; receptor-mediated endocytosis; platelet activation; cholesterol transport; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of tumor necrosis factor production; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; lipoprotein transport; toll-like receptor signaling pathway; innate immune response; lipid metabolic process; positive regulation of phagocytosis, engulfment; blood coagulation; nitric oxide mediated signal transduction; plasma membrane long-chain fatty acid transport; apoptotic cell clearance. Disease: Platelet Glycoprotein Iv Deficiency; Coronary Heart Disease, Susceptibility To, 7; Malaria, Susceptibility To

96 Tests

515 USD