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Glutaminase Microplate Assay Kit

MBS8243221

100 Assays

370 USD

Assay Kit

Glutaminase Microplate Assay Kit

[Glutaminase]

[EC 3.5.1.2; Glutaminase; L-glutamine amidohydrolase]

[glutaminase; Glutaminase liver isoform, mitochondrial; glutaminase liver isoform, mitochondrial; glutaminase 2; L-glutaminase; L-glutamine amidohydrolase]

[GLS2; GLS2; GA; GLS; LGA; hLGA; GA; GLS]

GLSL_HUMAN

General

263

Shipped and store at 4 degree C for 6 months.

Functional Assay

Typical Testing Data/Standard Curve (for reference only)

Background/Introduction: Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme which plays a key role in the Pentose Phosphate Pathway (PPP). The PPP pathway supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Recently studies have found increased G6PDH activity in various human tumors and knockdown of this G6PDH decreases cell proliferation and enhances apoptosis in vitro. Therefore, targeting this key enzyme is essential for developing novel therapeutic approach for treatment/cure of cancer. In BioVision's G6PDH assay kit, Glucose-6-Phosphate is oxidized by glucose-6-phosphate dehydrogenase to generate 6-Phosphoglucono-delta-Lactone and form NADH, which reacts with a probe to produce a signal at OD 450 nm. In the presence of G6PDH inhibitor, these reactions are impeded, thus decreasing the rate and/or extent of generation of G6PDHdependent absorbance. Thus, this kit provides a sensitive, quick, and easy method for screening potential inhibitors of G6PDH. G6PDH Inhibitor Control is included to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed in less than 30 min.

6650608

AAF21934.1

23,055 Da

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (1270159); Arginine Biosynthesis Pathway (82943); Arginine Biosynthesis Pathway (306); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); D-Glutamine And D-glutamate Metabolism Pathway (82971); D-Glutamine And D-glutamate Metabolism Pathway (340); GABAergic Synapse Pathway (377263)

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family. Protein type: Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Amino Acid Metabolism - alanine, aspartate and glutamate; Hydrolase; Amino Acid Metabolism - arginine and proline; Energy Metabolism - nitrogen; Mitochondrial; EC 3.5.1.2. Chromosomal Location of Human Ortholog: 12q13.3. Cellular Component: mitochondrial matrix; mitochondrion. Molecular Function: glutaminase activity; protein binding. Biological Process: amino acid biosynthetic process; amino acid metabolic process; gene expression; glutamate biosynthetic process; glutamate secretion; glutamine catabolic process; neurotransmitter secretion; regulation of apoptosis; synaptic transmission; transcription initiation from RNA polymerase II promoter

100 Assays

370 USD