catalog number :
MBS8243219
products type :
Assay Kit
products full name :
Alpha-Amylase Microplate Assay Kit
products short name :
[Alpha-Amylase]
products name syn :
[EC 3.2.1.1; Alpha-amylase; Glycogenase; A-Amylase]
other names :
[alpha-amylase; Pancreatic alpha-amylase; pancreatic alpha-amylase; amylase, alpha 2A (pancreatic); 1,4-alpha-D-glucan glucanohydrolase]
other gene names :
[AMY2A; AMY2A; PA; AMY2; PA]
uniprot entry name :
AMYP_HUMAN
storage stability :
Shipped and store at 4 degree C for 6 months.
tested application :
Functional Assay
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Urine, Saliva, Cell Culture, Tissue Extracts, Cell Lysate, Other Biological Fluids Samples. Detection Range: 1 umol/L-1000 umol/L
products description :
Background/Introduction: Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). Ammonia/Ammonium Microplate Assay Kit is designed to directly measure NH3 and NH4+ in a variety of samples. In the assay, ammonia reacts with hypochlorous acid, which is determined at 620nm, is directly proportional to the Ammonia/Ammonium concentration in the sample.
ncbi acc num :
AAA52280.1
ncbi mol weight :
30,302 Da
ncbi pathways :
Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); Digestion Of Dietary Carbohydrate Pathway (1269958); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Starch And Sucrose Metabolism Pathway (82974); Starch And Sucrose Metabolism Pathway (344)
ncbi summary :
This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015]
uniprot summary :
AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]. Protein type: EC 3.2.1.1; Hydrolase; Carbohydrate Metabolism - starch and sucrose; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1p21. Cellular Component: extracellular region; extracellular space. Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding. Biological Process: carbohydrate catabolic process; carbohydrate metabolic process; polysaccharide digestion