catalog number :
MBS8243214
products type :
Assay Kit
products full name :
Nitric Oxide Microplate Assay Kit
products short name :
[Nitric Oxide]
products name syn :
[Nitric Oxide; Nitrogen oxide; Nitrogen monoxide]
storage stability :
Shipped and store at 4 degree C for 6 months.
tested application :
Functional Assay
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Tissue Extracts, Cell Lysate Samples. Detection Range: 0.1 umol/ml-10 umol/ml
products description :
Background/Introduction: Nitrate Reductase (NR), also known as respiratory nitrate reductase. Eukaryotic nitrate reductases are part of the sulfite oxidase family of molybdoenzymes. They transfer electrons from NADH or NADPH to nitrate. Prokaryotic nitrate reductases belong to the DMSO reductase family of molybdoenzymes and have been classified into three groups, assimilatory nitrate reductases (Nas), respiratory nitrate reductase (Nar), and periplasmic nitrate reductases (Nap). The active site of these enzymes is a Mo ion that is bound to the four thiolate functions of two pterin molecules. The coordination sphere of the Mo is completed by one amino-acid side chain and oxygen and/or sulfur ligands. The exact environment of the Mo ion in certain of these enzymes (oxygen versus sulfur as a sixth molybdenum ligand) is still debated. The Mo is covalently attached to the protein by a cysteine ligand in Nap, and an aspartate in Nar. Nitrate reductase activity can be used as a biochemical tool for predicting grain yield and grain protein production. Nitrate reductase promotes amino acid production in tea leaves. It is reported that tea plants sprayed with various micronutrients (like Zn, Mn and B) along with Mo enhanced the amino acid content of tea shoots and also the crop yield. The assay is initiated with the enzymatic hydrolysis of the nitrate by Nitrate Reductase. The enzyme catalysed reaction products azo-compound can be measured at a colorimetric readout at 540 nm.