catalog number :
MBS8243212
products type :
Assay Kit
products full name :
Isocitrate Lyase Microplate Assay Kit
products short name :
[Isocitrate Lyase]
products name syn :
[EC 4.1.3.1; Isocitrate lyase; ICL; Isocitrase; Isocitratase; Isocitrate glyoxylate-lyase; Isocitritase]
other names :
[isocitrate lyase; Isocitrate lyase; isocitrate lyase; IsocitraseCurated; IsocitratsysaseCurated]
other gene names :
[ICL; ICL; isocitrate lyase; ICL]
uniprot entry name :
ACEA_ARATH
storage stability :
Shipped and store at 4 degree C for 6 months.
tested application :
Functional Assay
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Tissue Extracts, Cell Lysate, Cell Culture Media And Other Biological Fluids Samples. Detection Range: 0.2 mmol/L-20 mmol/L
products description :
Background/Introduction: Isocitrate Dehydrogenase (IDH) (EC 1.1.1.42) and (EC 1.1.1.41) is an enzyme which catalyzes the interconversion of isocitrate and alpha-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. Isocitrate Dehydrogenase Microplate Assay Kit is a sensitive assay for determining Isocitrate Dehydrogenase activity in various samples. Isocitrate Dehydrogenase activity is determined by the product of alpha-ketoglutarate. The increase in absorbance at 410 nm is directly proportional to the enzyme activity.
ncbi acc num :
NP_188809.2
ncbi gb acc num :
NM_113067.3
ncbi mol weight :
64,245 Da
ncbi pathways :
Biosynthesis Of Secondary Metabolites Pathway (148695); Carbon Metabolism Pathway (815031); Carbon Metabolism Pathway (817567); Glyoxylate And Dicarboxylate Metabolism Pathway (4057); Glyoxylate And Dicarboxylate Metabolism Pathway (383); Glyoxylate Cycle Pathway (382689); Glyoxylate Cycle Pathway (468205); Metabolic Pathways (132014); Seed Development Pathway (755444); Seed Development Pathway (755443)
ncbi summary :
Encodes a glyoxylate cycle enzyme isocitrate lyase (ICL).
uniprot summary :
Involved in storage lipid mobilization during the growth of higher plant seedling.