catalog number :
MBS8243203
products type :
Assay Kit
products full name :
Cellulase Microplate Assay Kit
products short name :
[Cellulase]
products name syn :
[EC 3.2.1.4; Cellulase; Avicelase; Beta-1,4-endoglucan hydrolase; Beta-1,4-glucanase; Carboxymethyl cellulase; Celludextrinase; Endo-1,4-beta-D-glucanase; Endo-1,4-beta-D-glucanohydrolase; Endo-1,4-beta-glucanase; Endoglucanase]
other names :
[cellulase; Cellulase; Cellulase]
other gene names :
[celMytA]
uniprot entry name :
G9MD89_9GAMM
storage stability :
Shipped and store at 4 degree C for 6 months.
tested application :
Functional Assay
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
products description :
Background/Introduction: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). CytoSelect Cell Migration Assay Kit utilizes polycarbonate membrane inserts (5 um pore size) to assay the migratory properties of cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-migratory cells (i.e. cotton swabbing). Any migratory cells are first dissociated from the membrane, then lysed and detected by the patented CyQuant GR Dye (Invitrogen). CytoSelect Cell Migration Assay Kit provides a robust system for the quantitative determination of cell migration. The kit contains sufficient reagents for the evaluation of 12 samples. The 5 um pore size is optimal for monocyte and macrophage cell migration. However, in the case of epithelial and fibroblast, a larger pore size (8 um) is recommended. For neutrophil chemotaxis, a smaller pore size (3 um) is recommended. The CytoSelect Cell Migration Assay Kit contains PET membrane inserts (5 um pore size) in a 24-well plate. The membrane serves as a barrier to discriminate migratory cells from non-migratory cells. Migratory cells are able to extend protrusions towards chemoattractants (via actin cytoskeleton reorganization) and ultimately pass through the pores of the polycarbonate membrane. These migratory cells are then dissociated from the membrane and subsequently detected by the patented CyQuant GR Dye (Invitrogen).
ncbi acc num :
BAL42331.1
ncbi mol weight :
127,562 Da