catalog number :
MBS8243199
products type :
Assay Kit
products full name :
Thioredoxin Reductase Microplate Assay Kit
products short name :
[Thioredoxin Reductase]
products name syn :
[EC 1.8.1.9; TR; TrxR; Thioredoxin-disulfide reductase; NADP-thioredoxin reductase; NADPH-thioredoxin reductase; NADPH:oxidized thioredoxin oxidoreductase; Thioredoxin reductase (NADPH)]
other names :
[thioredoxin reductase; thioredoxin reductase]
other gene names :
[trxB]
storage stability :
Shipped and store at 4 degree C for 6 months.
tested application :
Functional Assay
other info1 :
Samples: Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples
products description :
Background: Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Thioredoxin Reductase Microplate Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5’-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (?max = 412 nm).
ncbi acc num :
NP_864441.1
ncbi gb acc num :
NC_005027.1
ncbi pathways :
Pyrimidine Metabolism Pathway (13225); Pyrimidine Metabolism Pathway (309); Selenocompound Metabolism Pathway (13252); Selenocompound Metabolism Pathway (338)