catalog number :
MBS8243195
products type :
Assay Kit
products full name :
Glutathione Reductase Microplate Assay Kit
products short name :
[Glutathione Reductase]
products name syn :
[EC 1.8.1.7; GR; Glutathione-disulfide reductase; Glutathione reductase; Glutathione S-reductase; GSH reductase; GSSG reductase; NADPH-glutathione reductase; NADPH-GSSG reductase; NADPH:oxidized-glutathione oxidoreductase]
other names :
[glutathione reductase; Glutathione reductase, chloroplastic; glutathione reductase; Protein EMBRYO DEFECTIVE 2360]
other gene names :
[GR; EMB2360; ATGR2; EMB2360; glutathione reductase; GR; GR; GRase]
uniprot entry name :
GSHRP_ARATH
storage stability :
Shipped and store at 4 degree C for 6 months.
tested application :
Functional Assay
other info1 :
Samples: Urine, Serum, Plasma, Other biological fluids, Tissue extracts, Cell lysate, Cell culture media Samples
products description :
Background: Glutathione reductase (GR, EC 1.6.4.2) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to glutathione (GSH).This enzyme is essential for the GSH redox cycle which maintains adequate levels of reduced cellular GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. Glutathione Reductase Microplate Assay Kitmeasures GR activity by measuring the rate of NADPH oxidation. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. Since GR is present at rate limiting concentrations, the rate of decrease in the A340 is directly proportional to the GR activity in the sample.
ncbi acc num :
NP_191026.1
ncbi gb acc num :
NM_115323.3
ncbi mol weight :
60,852 Da
ncbi pathways :
Cellular Responses To Stress Pathway (1330688); Detoxification Of Reactive Oxygen Species Pathway (1330689); Gene Expression Pathway (1330623); Generic Transcription Pathway (1330624); Glutathione Metabolism Pathway (4032); Glutathione Metabolism Pathway (343); Metabolism Pathway (1331013); Metabolism Of Amino Acids And Derivatives Pathway (1331175); Metabolism Of Ingested MeSeO2H Into MeSeH Pathway (1331203); Metabolism Of Nucleotides Pathway (1331150)
ncbi summary :
Encodes glutathione reductase that is most likely localized in the chloroplast.
uniprot summary :
Maintains high levels of reduced glutathione in the chloroplast.