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Glutamine Synthetase Microplate Assay Kit

MBS8243181

100 Assays

285 USD

Assay Kit

Glutamine Synthetase Microplate Assay Kit

[Glutamine Synthetase]

[EC 6.3.1.2; Glutamate-ammonia ligase; Glutamine synthetase; GS]

[glutamine synthetase; Glutamine synthetase; glutamine synthetase; glutamate-ammonia ligase; Glutamate decarboxylase (EC:4.1.1.15); Glutamate--ammonia ligase]

[GLUL; GLUL; GS; GLNS; PIG43; PIG59; GLNS; GS]

GLNA_HUMAN

General

373

Shipped and store at 4 degree C for 6 months.

Functional Assay

Typical Testing Data/Standard Curve (for reference only)

Samples: Tissue Extracts, Cell Lysate Samples. Detection Range: 10 umol/L-500 umol/L

Background/Introduction: Glutaminase (glutamine aminohydrolase or GLS) catalyzes the following reaction: Glutamine + H2O --> Glutamate + NH3. The enzyme has tissue-specific roles in multiple organs. Two different mammalian phosphate-activated GLS isoforms are known: GLS1 (kidney-type) and GLS2 (liver-type; a target of the tumor suppressor protein p53). The hydrolytic activity of glutaminase generates ammonia for urea synthesis in the liver similar to that mediated by glutamate dehydrogenase. During renal acidosis, glutaminase is induced in the kidney, leading to increased excretion of ammonia, which plays an important role in maintaining acid-base homeostasis. Glutaminase regulates the levels of the neurotransmitter glutamate in the brain. The rate of glutaminolysis is known to increase in tumors and may be a hot spot for regulation of cancer cell metabolism. Inhibitors of GLS may therefore be candidate drugs for cancer therapy. The assay is initiated with the enzymatic hydrolysis of glutamine by GLS. The enzyme catalysed reaction products can be measured at a colorimetric readout at 420 nm.

74271837

NP_001028216.1

NM_001033044.3

42,064 Da

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (1270159); Arginine Biosynthesis Pathway (82943); Arginine Biosynthesis Pathway (306); Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway (1268785); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); GABA Shunt Pathway (142350); GABAergic Synapse Pathway (377263)

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family. Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; Energy Metabolism - nitrogen; EC 6.3.1.2; EC 4.1.1.15; Ligase. Chromosomal Location of Human Ortholog: 1q31. Cellular Component: cytoplasm; cytosol; mitochondrion; myelin sheath; nerve terminal; nucleus; perikaryon; protein complex; rough endoplasmic reticulum. Molecular Function: ATP binding; dynein light chain binding; glutamate binding; glutamate decarboxylase activity; glutamate-ammonia ligase activity; identical protein binding; magnesium ion binding; manganese ion binding. Biological Process: amino acid biosynthetic process; cell proliferation; cellular response to starvation; glutamate catabolic process; glutamine biosynthetic process; neurotransmitter uptake; positive regulation of epithelial cell proliferation; positive regulation of insulin secretion; positive regulation of synaptic transmission, glutamatergic; protein homooligomerization; response to glucose stimulus; synaptic transmission. Disease: Glutamine Deficiency, Congenital

100 Assays

285 USD