Anti-HEXIM1 Antibody | MyBioSource MBS8243164 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-HEXIM1 Antibody

catalog :

MBS8243164

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of HEXIM1 expression in A431 (A), Jurkat (B), NIH3T3 (C), rat heart (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of HEXIM1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of HEXIM1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8243164

products type :

Antibody

products full name :

Anti-HEXIM1 Antibody

products short name :

[HEXIM1]

products name syn :

[CLP1; EDG1; HIS1; MAQ1; Protein HEXIM1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1]

other names :

[Protein HEXIM1; Protein HEXIM1; protein HEXIM1; hexamethylene bis-acetamide inducible 1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1]

products gene name :

[HEXIM1]

other gene names :

[HEXIM1; HEXIM1; CLP1; EDG1; HIS1; MAQ1; CLP1; EDG1; HIS1; MAQ1]

uniprot entry name :

HEXI1_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat, Monkey

sequence length :

359

specificity :

Recognizes endogenous levels of HEXIM1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

Western Blot: (1/500 - 1/1000). Immunohistochemistry: (1/100 - 1/200). Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HEXIM1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to HEXIM1

ncbi gi num :

74735573

ncbi acc num :

O94992.1

uniprot acc num :

O94992

ncbi mol weight :

40,623 Da

ncbi summary :

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]

uniprot summary :

HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family. Protein type: Transcription factor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17q21.31. Cellular Component: cytoplasm; nucleoplasm; nucleus. Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding. Biological Process: heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage