Anti-SMRT Antibody | MyBioSource MBS8242952 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-SMRT Antibody

catalog :

MBS8242952

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of SMRT expression in HeLa (A), HEK293T (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of SMRT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of SMRT staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8242952

products type :

Antibody

products full name :

Anti-SMRT Antibody

products short name :

[SMRT]

products name syn :

[CTG26; Nuclear receptor corepressor 2; N-CoR2; CTG repeat protein 26; SMAP270; Silencing mediator of retinoic acid and thyroid hormone receptor; SMRT; T3 receptor-associating factor; TRAC; Thyroid-, retinoic-acid-receptor-associated corepressor]

other names :

[Nuclear receptor corepressor 2; Nuclear receptor corepressor 2; nuclear receptor corepressor 2; nuclear receptor corepressor 2; CTG repeat protein 26; SMAP270; Silencing mediator of retinoic acid and thyroid hormone receptor; SMRT; T3 receptor-associating factor; TRAC; Thyroid-, retinoic-acid-receptor-associated corepressor]

products gene name :

[NCOR2]

other gene names :

[NCOR2; NCOR2; SMRT; TRAC; CTG26; SMRTE; TRAC1; N-CoR2; TNRC14; TRAC-1; SMAP270; SMRTE-tau; CTG26; N-CoR2; SMRT; TRAC]

uniprot entry name :

NCOR2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

2525

specificity :

Recognizes endogenous levels of SMRT protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Ships on blue ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze-thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMRT. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to SMRT

ncbi gi num :

226713806

ncbi acc num :

Q9Y618.2

uniprot acc num :

Q9Y618

ncbi pathways :

Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806); Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (1268891); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (1268889); Delta-Notch Signaling Pathway (198879); Developmental Biology Pathway (1270302); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855)

ncbi summary :

This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Apr 2011]

uniprot summary :

Transcriptional corepressor. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival.

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage