Anti-Vasopressin V2 Receptor Antibody | MyBioSource MBS8242744 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Vasopressin V2 Receptor Antibody

catalog :

MBS8242744

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Vasopressin V2 Receptor expression in HEK293T (A), RAW264.7 (B), rat muscle (C) whole cell lysates.

image 2 :

Immunofluorescent analysis of Vasopressin V2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8242744

products type :

Antibody

products full name :

Anti-Vasopressin V2 Receptor Antibody

products short name :

[Vasopressin V2 Receptor]

products name syn :

[ADHR; DIR; DIR3; V2R; Vasopressin V2 receptor; V2R; AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor]

other names :

[Vasopressin V2 receptor; Vasopressin V2 receptor; vasopressin V2 receptor; arginine vasopressin receptor 2; AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor]

products gene name :

[AVPR2]

other gene names :

[AVPR2; AVPR2; DI1; DIR; NDI; V2R; ADHR; DIR3; ADHR; DIR; DIR3; V2R; V2R]

uniprot entry name :

V2R_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Monkey

sequence length :

371

specificity :

Recognizes endogenous levels of Vasopressin V2 Receptor protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Shipping: Blue ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Wester Blot (WB), Immunofluorescence (IF)/ Immunocytochemistry (ICC).

app notes :

WB 1/500-1/100. IF/ICC 1/100-1/500

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Vasopressin V2 Receptor. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Vasopressin V2 Receptor

ncbi gi num :

267256

ncbi acc num :

P30518.1

uniprot acc num :

P30518

ncbi pathways :

Aquaporin-mediated Transport Pathway (1269944); Arf6 Trafficking Events Pathway (137954); Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); Defective AVP Causes Neurohypophyseal Diabetes Insipidus (NDI) Pathway (1309124); Disease Pathway (1268854); Disorders Of Transmembrane Transporters Pathway (1268932); G Alpha (s) Signalling Events Pathway (1269575); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886)

ncbi summary :

This gene encodes the vasopressin receptor, type 2, also known as the V2 receptor, which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily, and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor, the V1a and V1b vasopressin receptors, the oxytocin receptor, and isotocin and mesotocin receptors in non-mammals, is well conserved, though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts, where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost, the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these 'extrarenal receptors' are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. [provided by RefSeq, Jul 2008]

uniprot summary :

AVPR2: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption. Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD). This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI); also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: GPCR, family 1; Receptor, GPCR; Transporter; Membrane protein, multi-pass; Transporter, aquaporin family; Membrane protein, integral. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: endoplasmic reticulum; endosome; Golgi apparatus; integral to membrane; integral to plasma membrane; plasma membrane. Molecular Function: peptide binding; protein binding; vasopressin receptor activity. Biological Process: adenylate cyclase activation; cellular response to hormone stimulus; excretion; G-protein signaling, coupled to cAMP nucleotide second messenger; hemostasis; I-kappaB kinase/NF-kappaB cascade; interferon-gamma production; positive regulation of cell proliferation; positive regulation of protein ubiquitination; positive regulation of systemic arterial blood pressure; positive regulation of vasoconstriction; regulation of systemic arterial blood pressure by vasopressin; renal water homeostasis; response to cytokine stimulus; telencephalon development; transmembrane transport; water transport. Disease: Diabetes Insipidus, Nephrogenic, X-linked; Nephrogenic Syndrome Of Inappropriate Antidiuresis

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage