Anti-DNA Polymerase zeta Antibody | MyBioSource MBS8242586 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-DNA Polymerase zeta Antibody

catalog :

MBS8242586

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of DNA Polymerase zeta expression in K562 (A), NIH3T3 (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of DNA Polymerase zeta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of DNA Polymerase zeta staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8242586

products type :

Antibody

products full name :

Anti-DNA Polymerase zeta Antibody

products short name :

[DNA Polymerase zeta]

products name syn :

[POLZ; REV3; DNA polymerase zeta catalytic subunit; Protein reversionless 3-like; REV3-like; hREV3]

other names :

[DNA polymerase zeta catalytic subunit; DNA polymerase zeta catalytic subunit; DNA polymerase zeta catalytic subunit; REV3 like, DNA directed polymerase zeta catalytic subunit; Protein reversionless 3-like; REV3-like; hREV3]

products gene name :

[REV3L]

other gene names :

[REV3L; REV3L; POLZ; REV3; POLZ; REV3; REV3-like; hREV3]

uniprot entry name :

DPOLZ_HUMAN

clonality :

polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat, Bovine, Chicken, Dog, Pig

sequence length :

3130

specificity :

Recognizes endogenous levels of DNA Polymerase zeta protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Do not freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Polymerase zeta. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to DNA Polymerase zeta

ncbi gi num :

143811385

ncbi acc num :

O60673.2

uniprot acc num :

O60673

ncbi mol weight :

344,154 Da

ncbi pathways :

DNA Damage Bypass Pathway (1270367); DNA Repair Pathway (1270350); DNA Polymerase Zeta Complex Pathway (413480); DNA Polymerase Zeta Complex Pathway (468391); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Metabolic Pathways (132956); Translesion Synthesis By POLI Pathway (1270373); Translesion Synthesis By POLK Pathway (1270372); Translesion Synthesis By REV1 Pathway (1270370)

uniprot summary :

REV3: the DNA polymerase zeta catalytic subunit. Catalyzes the production of N diphosphate + {DNA}N from N deoxynucleoside triphosphate. Involved in translesion synthesis and required for the production of UV- and other DNA fork-blocking-induced mutations, as well as the majority of spontaneous mutations. Protein type: Transferase; EC 2.7.7.7; DNA replication. Chromosomal Location of Human Ortholog: 6q21. Cellular Component: nucleolus; nucleoplasm; zeta DNA polymerase complex. Molecular Function: 3'-5' exonuclease activity; 4 iron, 4 sulfur cluster binding; DNA binding; DNA-directed DNA polymerase activity; metal ion binding; nucleotide binding; protein binding. Biological Process: bypass DNA synthesis; DNA repair; DNA-dependent DNA replication; error-prone postreplication DNA repair

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage