Anti-KSR1 Antibody | MyBioSource MBS8242341 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-KSR1 Antibody

catalog :

MBS8242341

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of KSR1 expression in HEK293T (A), NIH3T3 (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of KSR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of KSR1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8242341

products type :

Antibody

products full name :

Anti-KSR1 Antibody

products short name :

[KSR1]

products name syn :

[KSR; Kinase suppressor of Ras 1]

other names :

[Kinase suppressor of Ras 1; Kinase suppressor of Ras 1; kinase suppressor of Ras 1; kinase suppressor of ras 1]

products gene name :

[KSR1]

other gene names :

[KSR1; KSR1; KSR; RSU2; KSR]

uniprot entry name :

KSR1_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat

specificity :

Recognizes endogenous levels of KSR1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Shipped and store at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human KSR1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to KSR1

ncbi gi num :

677286751

ncbi acc num :

Q8IVT5.3

uniprot acc num :

Q8IVT5

ncbi mol weight :

84,697 Da

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); Axon Guidance Pathway (1270303); BDNF Signaling Pathway (712093); Ceramide Signaling Pathway (138023); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Developmental Biology Pathway (1270302); Downstream Signal Transduction Pathway (1269479); Downstream Signaling Of Activated FGFR1 Pathway (1269392)

uniprot summary :

KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described. Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RAF family. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; membrane; protein complex. Molecular Function: ATP binding; MAP-kinase scaffold activity; metal ion binding; mitogen-activated protein kinase kinase binding; protein binding; protein C-terminus binding; protein kinase activity. Biological Process: activation of MAPKK activity; axon guidance; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage