Anti-FOXO1 (pS329) Antibody | MyBioSource MBS8242325 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-FOXO1 (pS329) Antibody

catalog :

MBS8242325

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of FOXO1 (pS329) expression in HEK293T (A), SHSY5Y (B), NIH3T3 (C), mouse brain (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of FOXO1 (pS329) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of FOXO1 (pS329) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8242325

products type :

Antibody

products full name :

Anti-FOXO1 (pS329) Antibody

products short name :

[FOXO1]

products name syn :

[FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma]

other names :

[Forkhead box protein O1; Forkhead box protein O1; forkhead box protein O1; forkhead box O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma]

products gene name :

[FOXO1]

other gene names :

[FOXO1; FOXO1; FKH1; FKHR; FOXO1A; FKHR; FOXO1A]

uniprot entry name :

FOXO1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Pig

sequence length :

655

specificity :

Recognizes endogenous levels of FOXO1 (pS329) protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Shipped at 4 degree C. Upon delivery and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to FOXO1 (pS329)

ncbi gi num :

116241368

ncbi acc num :

Q12778.2

uniprot acc num :

Q12778

ncbi pathways :

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); AGE/RAGE Pathway (698754); AKT Phosphorylates Targets In The Nucleus Pathway (1269190); AKT-mediated Inactivation Of FOXO1A Pathway (1270342); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adaptive Immune System Pathway (1269171); Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806)

ncbi summary :

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

uniprot summary :

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain. Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 13q14.1. Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus. Molecular Function: beta-catenin binding; chromatin binding; protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding. Biological Process: anatomical structure morphogenesis; apoptosis; autophagy; blood vessel development; cell differentiation; cell glucose homeostasis; cellular response to insulin stimulus; cellular response to starvation; endocrine pancreas development; epidermal growth factor receptor signaling pathway; fat cell differentiation; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of gluconeogenesis; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid acetylation; response to DNA damage stimulus; thermoregulation; transcription, DNA-dependent. Disease: Rhabdomyosarcoma 2

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage