Anti-MARCKS Antibody | MyBioSource MBS8242144 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-MARCKS Antibody

catalog :

MBS8242144

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, cow

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of MARCKS expression in Jurkat (A), mouse brain (B), PC12 (C) whole cell lysates.

image 2 :

Immunofluorescent analysis of MARCKS staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8242144

products type :

Antibody

products full name :

Anti-MARCKS Antibody

products short name :

[MARCKS]

products name syn :

[MACS; PRKCSL; Myristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; PKCSL]

other names :

[Myristoylated alanine-rich C-kinase substrate; Myristoylated alanine-rich C-kinase substrate; myristoylated alanine-rich C-kinase substrate; myristoylated alanine rich protein kinase C substrate; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; PKCSL]

products gene name :

[MARCKS]

other gene names :

[MARCKS; MARCKS; MACS; 80K-L; PKCSL; PRKCSL; MACS; PRKCSL; MARCKS; 80K-L protein; PKCSL]

uniprot entry name :

MARCS_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Chicken, Zebrafish

sequence length :

332

specificity :

Recognizes endogenous levels of MARCKS protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

Western Blot: (1/500 - 1/1000). Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MARCKS. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to MARCKS

ncbi gi num :

76803798

ncbi acc num :

P29966.4

uniprot acc num :

P29966

ncbi pathways :

Acetylcholine Regulates Insulin Secretion Pathway (1270104); BDNF Signaling Pathway (712093); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590); Integration Of Energy Metabolism Pathway (1270101); Metabolism Pathway (1269956); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Regulation Of Insulin Secretion Pathway (1270102)

ncbi summary :

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

uniprot summary :

MARCKS: the most prominent cellular substrate of protein kinase C. Binds calmodulin, actin, and synapsin. A filamentous actin cross-linking protein. Critical for normal brain development. Phosphorylation by PKC displaces it from the plasma membrane, inhibiting F-actin cross-linking activity. Plays a role in growth cone adhesion: its nonphosphorylated form stabilizes integrin-mediated adhesions, and its phosphorylation-triggered release from adhesions causes localized growth cone detachment. Highly expressed in immature T and B lymphocytes: its expression is highest in CD4-8- T cells and B-cell blasts generated by antigen receptor signaling. Its expression and phosphorylation are closely linked to microglial activation processes involved in pathological conditions such as neuroinflammation and neurodegeneration. Protein type: Motility/polarity/chemotaxis; Actin-binding. Chromosomal Location of Human Ortholog: 6q22.2. Cellular Component: actin cytoskeleton; cell cortex; centrosome; cytosol; dendritic spine; focal adhesion; germinal vesicle; growth cone; lysosome; microtubule; nerve terminal; outer dense fiber; plasma membrane. Molecular Function: actin filament binding; calmodulin binding; phosphatidylserine binding; protein kinase C binding. Biological Process: actin filament organization; brain development; cell-substrate adhesion; energy reserve metabolic process; intracellular protein transport; phospholipase D activation; regulation of insulin secretion

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage