Anti-LATS1/2 Antibody | MyBioSource MBS8241758 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-LATS1/2 Antibody

catalog :

MBS8241758

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of LATS1/2 expression in K562 (A), Hela (B), HEK293T (C), mouse lung (D), rat muscle (E) whole cell lysates.

product information

catalog number :

MBS8241758

products type :

Antibody

products full name :

Anti-LATS1/2 Antibody

products short name :

[LATS1/2]

products name syn :

[LATS1; WARTS; Serine/threonine-protein kinase LATS1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts; LATS2; KPM; Serine/threonine-protein kinase LATS2; Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; Serine/threonine-protein kinase kpm; Warts-like kinase]

other names :

[Serine/threonine-protein kinase LATS1; Serine/threonine-protein kinase LATS1; serine/threonine-protein kinase LATS1; large tumor suppressor kinase 1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts]

products gene name :

[LATS1]

products gene name syn :

[LATS2]

other gene names :

[LATS1; LATS1; wts; WARTS; h-warts]

uniprot entry name :

LATS1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

1130

specificity :

Recognizes endogenous levels of LATS1/2 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

storage stability :

Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB)

app notes :

Western Blot: (1/500 - 1/1000)

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human LATS1/2. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to LATS1/2

ncbi gi num :

52783106

ncbi acc num :

O95835.1

uniprot acc num :

O95835

ncbi pathways :

Hippo Signaling Pathway (1084759); Hippo Signaling Pathway (1108222); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Signal Transduction Pathway (1269379); Signaling By Hippo Pathway (1269616)

ncbi summary :

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. Two protein-coding transcripts and one non-protein coding transcript have been found for this gene. [provided by RefSeq, Jul 2012]

uniprot summary :

LATS1: an AGC kinase of the NDR family of kinases. Localized to the mitotic apparatus and specifically phosphorylated during mitosis. A likely tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDC2 kinase activity, causing G2 arrest. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. Complexes with CDC2 in early mitosis. LATS1-associated CDC2 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated zyxin, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Knockout mice are susceptible to soft-tissue sarcomas and sensitive to chemical carcinogenesis. Human soft tissue sarcomas have downregulated, mutated, and/or hypermethylated LATS1. Transgenic expression blocks anchorage independent growth in culture and tumor growth in xenografts. Two alternatively spliced isoforms of the human proteinhave been described. Protein type: Protein kinase, AGC; Tumor suppressor; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; NDR family. Chromosomal Location of Human Ortholog: 6q25.1. Cellular Component: cytosol; microtubule organizing center; spindle pole. Molecular Function: ATP binding; magnesium ion binding; protein binding; protein kinase binding; protein serine/threonine kinase activity. Biological Process: cell division; cytoplasmic sequestering of protein; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; hormone-mediated signaling; inner cell mass cell fate commitment; inner cell mass cellular morphogenesis; keratinocyte differentiation; mitosis; negative regulation of cyclin-dependent protein kinase activity; peptidyl-serine phosphorylation; positive regulation of apoptosis; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of actin filament polymerization; regulation of organ growth; regulation of protein complex assembly; sister chromatid segregation

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage