product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-NT5C1A Antibody
catalog :
MBS8241620
quantity :
0.03 mL
price :
155 USD
clonality :
polyclonal
host :
unidentified
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry
more info or order :
image
image 1 :
MyBioSource MBS8241620 image 1
Western blot analysis of NT5C1A expression in HepG2 (A), mouse brain (B), rat muscle (C) whole cell lysates.
image 2 :
MyBioSource MBS8241620 image 2
Immunofluorescent analysis of NT5C1A staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
product information
catalog number :
MBS8241620
products type :
Antibody
products full name :
Anti-NT5C1A Antibody
products short name :
[NT5C1A]
products name syn :
[Cytosolic 5'-nucleotidase 1A; cN1A; Cytosolic 5'-nucleotidase IA; cN-I; cN-IA]
other names :
[Cytosolic 5'-nucleotidase 1A; Cytosolic 5'-nucleotidase 1A; cytosolic 5'-nucleotidase 1A; 5'-nucleotidase, cytosolic IA; Cytosolic 5'-nucleotidase IA; cN-I; cN-IA]
products gene name :
[NT5C1A]
other gene names :
[NT5C1A; NT5C1A; CN1; CNI; CN-I; CN1A; CN-IA; cN1A; cN-I; cN-IA]
uniprot entry name :
5NT1A_HUMAN
clonality :
Polyclonal
host :
unknown
reactivity :
Human, Mouse, Rat
sequence length :
368
specificity :
Recognizes endogenous levels of NT5C1A protein.
purity :
The antibody was purified by immunogen affinity chromatography.
form :
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
storage stability :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
tested application :
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
app notes :
Western Blot: (1/500 - 1/1000); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)
image1 heading :
Western Blot (WB)
image2 heading :
Immunofluorescence (IF)
other info1 :
Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NT5C1A. The exact sequence is proprietary.
products description :
Rabbit polyclonal antibody to NT5C1A
ncbi gi num :
47116736
ncbi acc num :
Q9BXI3.1
uniprot acc num :
Q9BXI3
ncbi mol weight :
41,021 Da
ncbi pathways :
Integrated Pancreatic Cancer Pathway (711360); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Nucleotides Pathway (1270133); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (1270137); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (1270134); Purine Metabolism Pathway (307)
ncbi summary :
Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008]
uniprot summary :
NT5C1A: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia. Belongs to the 5'-nucleotidase type 3 family. Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 1p34.3-p33. Cellular Component: cytosol. Molecular Function: 5'-nucleotidase activity; magnesium ion binding; nucleotide binding. Biological Process: adenosine metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; nucleoside metabolic process; purine base metabolic process; purine nucleoside monophosphate catabolic process; purine nucleotide catabolic process; pyrimidine base metabolic process; pyrimidine nucleoside catabolic process
size1 :
0.03 mL
price1 :
155 USD
size2 :
0.1 mL
price2 :
220
size3 :
0.2 mL
price3 :
295
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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