product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-eIF4AI Antibody
catalog :
MBS8241535
quantity :
0.03 mL
price :
155 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of eIF4AI expression in 293T (A), Hela (B), HepG2 (C), mouse brain (D) whole cell lysates.
image 2 :
Immunohistochemical analysis of eIF4AI staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
image 3 :
Immunofluorescent analysis of eIF4AI staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
product information
catalog number :
MBS8241535
products type :
Antibody
products full name :
Anti-eIF4AI Antibody
products short name :
[eIF4AI]
products name syn :
[DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1]
other names :
[Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4A-I; eukaryotic initiation factor 4A-I; eukaryotic translation initiation factor 4A1; ATP-dependent RNA helicase eIF4A-1]
products gene name :
[EIF4A1]
other gene names :
[EIF4A1; EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I; DDX2A; EIF4A; eIF-4A-I; eIF4A-I]
uniprot entry name :
IF4A1_HUMAN
clonality :
Monoclonal
host :
Mouse
reactivity :
Human, Mouse, Rat
sequence length :
406
specificity :
Recognizes endogenous levels of eIF4AI protein.
form :
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
storage stability :
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
tested application :
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
app notes :
Western Blot: (1/1000 - 1/3000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/200)
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Immunofluorescence (IF)
other info1 :
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human eIF4AI. The exact sequence is proprietary.
products description :
Mouse monoclonal to eIF4AI
ncbi gi num :
46397463
ncbi acc num :
P60842.1
uniprot acc num :
P60842
ncbi mol weight :
39,548 Da
ncbi pathways :
Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (1268683); Antiviral Mechanism By IFN-stimulated Genes Pathway (1269316); Cap-dependent Translation Initiation Pathway (1268680); Cytokine Signaling In Immune System Pathway (1269310); Deadenylation Of MRNA Pathway (1269713); Deadenylation-dependent MRNA Decay Pathway (1269712); Eukaryotic Translation Initiation Pathway (1268679); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (1268686); Gene Expression Pathway (1269649); ISG15 Antiviral Mechanism Pathway (1269317)
uniprot summary :
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
size1 :
0.03 mL
price1 :
155 USD
size2 :
0.1 mL
price2 :
220
size3 :
0.2 mL
price3 :
295
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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