Anti-BAF250B Antibody | MyBioSource MBS8241303 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-BAF250B Antibody

catalog :

MBS8241303

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of BAF250B expression in HeLa (A), HepG2 (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of BAF250B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of BAF250B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8241303

products type :

Antibody

products full name :

Anti-BAF250B Antibody

products short name :

[BAF250B]

products name syn :

[BAF250B; DAN15; KIAA1235; OSA2; AT-rich interactive domain-containing protein 1B; ARID domain-containing protein 1B; BRG1-associated factor 250b; BAF250B; BRG1-binding protein hELD/OSA1; Osa homolog 2; hOsa2; p250R]

other names :

[AT-rich interactive domain-containing protein 1B isoform 1; AT-rich interactive domain-containing protein 1B; AT-rich interactive domain-containing protein 1B; AT rich interactive domain 1B (SWI1-like); BRG1-associated factor 250b; BAF250B; BRG1-binding protein hELD/OSA1; Osa homolog 2; hOsa2; p250R]

products gene name :

[ARID1B]

other gene names :

[ARID1B; ARID1B; OSA2; 6A3-5; DAN15; MRD12; P250R; BRIGHT; BAF250B; ELD/OSA1; BAF250B; DAN15; KIAA1235; OSA2; ARID domain-containing protein 1B; BAF250B; hOsa2]

uniprot entry name :

ARI1B_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Porcine

sequence length :

1,486

specificity :

Recognizes endogenous levels of BAF250B protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000). IHC (1/100 - 1/200). IF/IC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAF250B. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to BAF250B

ncbi gi num :

297139703

ncbi acc num :

NP_059989.2

ncbi gb acc num :

NM_017519.2

uniprot acc num :

Q8NFD5

ncbi pathways :

Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433); Prostate Cancer Pathway (755440); RMTs Methylate Histone Arginines Pathway (1270439)

ncbi summary :

This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2012]

uniprot summary :

ARID1B: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Binds DNA non-specifically. Defects in ARID1B are the cause of mental retardation autosomal dominant type 12 (MRD12). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD12 patients present with moderate to severe psychomotor retardation, and most show evidence of muscular hypotonia. In many patients, expressive speech is more severely affected than receptive function. Additional common findings include short stature, abnormal head shape and low-set, posteriorly rotated, and abnormally shaped ears, downslanting palpebral fissures, a bulbous nasal tip, a thin upper lip, minor teeth anomalies, and brachydactyly or single palmar creases. Autistic features are uncommon. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor co-regulator; DNA-binding. Chromosomal Location of Human Ortholog: 6q25.1. Cellular Component: nucleoplasm; SWI/SNF complex; cytoplasm. Molecular Function: protein binding; DNA binding; transcription coactivator activity. Biological Process: nervous system development; transcription, DNA-dependent; chromatin-mediated maintenance of transcription. Disease: Mental Retardation, Autosomal Dominant 12

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage