Anti-Histone Deacetylase 6 (pS22) Antibody | MyBioSource MBS8240627 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Histone Deacetylase 6 (pS22) Antibody

catalog :

MBS8240627

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Histone Deacetylase 6 (pS22) expression in HepG2 (A), HeLa (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of Histone Deacetylase 6 (pS22) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Histone Deacetylase 6 (pS22) staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8240627

products type :

Antibody

products full name :

Anti-Histone Deacetylase 6 (pS22) Antibody

products short name :

[Histone Deacetylase 6]

products name syn :

[KIAA0901; Histone deacetylase 6; HD6]

other names :

[histone deacetylase 6; Histone deacetylase 6; histone deacetylase 6; histone deacetylase 6]

products gene name :

[HDAC6]

other gene names :

[HDAC6; HDAC6; HD6; JM21; CPBHM; PPP1R90; KIAA0901; HD6]

uniprot entry name :

HDAC6_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Monkey

sequence length :

1215

specificity :

Recognizes endogenous levels of Histone Deacetylase 6 (pS22) protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone Deacetylase 6 (pS22). The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Histone Deacetylase 6 (pS22)

ncbi gi num :

13128864

ncbi acc num :

NP_006035.2

ncbi gb acc num :

NM_006044.2

uniprot acc num :

Q9UBN7

ncbi mol weight :

114,361 Da

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Assembly Of The Primary Cilium Pathway (1268846); Cell Cycle Pathway (198811); Cellular Response To Heat Stress Pathway (1270421); Cellular Responses To Stress Pathway (1270414); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (1268891); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (1268889); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855)

ncbi summary :

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

uniprot summary :

HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily. Protein type: Ubiquitin conjugating system; EC 3.5.1.98; Nuclear receptor co-regulator; Deacetylase. Chromosomal Location of Human Ortholog: Xp11.23. Cellular Component: microtubule; histone deacetylase complex; dendrite; leading edge; perikaryon; inclusion body; caveola; cytosol; nucleoplasm; dynein complex; microtubule associated complex; perinuclear region of cytoplasm; axon; cytoplasmic microtubule; cytoplasm; nucleus. Molecular Function: zinc ion binding; histone deacetylase binding; microtubule binding; beta-catenin binding; beta-tubulin binding; Hsp90 protein binding; misfolded protein binding; actin binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; tubulin deacetylase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); ubiquitin protein ligase binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; polyubiquitin binding; tau protein binding; alpha-tubulin binding. Biological Process: negative regulation of proteolysis; response to misfolded protein; protein polyubiquitination; positive regulation of signal transduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; transcription, DNA-dependent; negative regulation of microtubule depolymerization; regulation of fat cell differentiation; macroautophagy; organelle organization and biogenesis; response to toxin; misfolded or incompletely synthesized protein catabolic process; histone deacetylation; regulation of gene expression, epigenetic; response to organic substance; intracellular protein transport; protein complex disassembly; lysosome localization; protein amino acid deacetylation; negative regulation of oxidoreductase activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent; negative regulation of protein complex disassembly. Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage