Anti-BAF53A Antibody | MyBioSource MBS8239389 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-BAF53A Antibody

catalog :

MBS8239389

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of BAF53A expression in K562 (A), Raw264.7 (B), PC12 (C), H9C2 (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of BAF53A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of BAF53A staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8239389

products type :

Antibody

products full name :

Anti-BAF53A Antibody

products short name :

[BAF53A]

products name syn :

[BAF53; BAF53A; INO80K; Actin-like protein 6A; 53 kDa BRG1-associated factor A; Actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF53A; INO80 complex subunit K]

other names :

[actin-like protein 6A isoform 1; Actin-like protein 6A; actin-like protein 6A; actin-like 6A; 53 kDa BRG1-associated factor A; Actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF53A; INO80 complex subunit K]

products gene name :

[ACTL6A]

other gene names :

[ACTL6A; ACTL6A; Arp4; ACTL6; BAF53A; INO80K; ARPN-BETA; BAF53; BAF53A; INO80K; BAF53A]

uniprot entry name :

ACL6A_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Chicken, Dog, Porcine

sequence length :

429

specificity :

Recognizes endogenous levels of BAF53A protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAF53A. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to BAF53A

ncbi gi num :

4757718

ncbi acc num :

NP_004292.1

ncbi gb acc num :

NM_004301.4

uniprot acc num :

O96019

ncbi pathways :

C-MYC Pathway (169344); Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433); Gastric Cancer Network 1 Pathway (760635); HATs Acetylate Histones Pathway (1270435); Prostate Cancer Pathway (755440); RMTs Methylate Histone Arginines Pathway (1270439); TNF-alpha/NF-kB Signaling Pathway (198884); Validated Targets Of C-MYC Transcriptional Activation Pathway (169351)

ncbi summary :

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

BAF53A: an actin-related protein involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of the helicase SMARCA4 and for association of the SMARCA4 containing remodelling complex BAF with chromatin/nuclear matrix. A component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of specific genes. Two alternatively spliced human isoforms have been described. Chromosomal Location of Human Ortholog: 3q26.33. Cellular Component: nucleoplasm; SWI/SNF complex; NuA4 histone acetyltransferase complex; protein complex; nuclear chromatin; plasma membrane; nucleus. Molecular Function: protein binding; nucleosomal DNA binding; transcription coactivator activity; chromatin binding. Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin remodeling; nervous system development; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of growth; ATP-dependent chromatin remodeling; DNA repair; signal transduction; DNA recombination

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage