gene knockdown

STAT3 siRNA (Human)

MBS8235655

15 nmol

365 USD

siRNA

STAT3 siRNA (Human)

[STAT3]

[APRF; Signal transducer and activator of transcription 3; Acute-phase response factor]

[signal transducer and activator of transcription 3 isoform 2; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); Acute-phase response factor]

[STAT3]

[STAT3; STAT3; APRF; HIES; ADMIO; APRF]

STAT3_HUMAN

Synthetic

Human

769

STAT3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

> 97%

Lyophilized powder

Shipped at 4 degree C. Store at -20 degree C for one year.

RNA Interference (RNAi)

Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We pre-designed sets containing 3 different target-specific siRNA oligo duplexes of human STAT3 gene MBS8235655-A, MBS8235655-B, MBS8235655-C. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

siRNA to inhibit STAT3 expression using RNA interference

21618338

NP_003141.2

NM_003150.3

P40763

AGE/RAGE Pathway (698754); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806); B Cell Receptor Signaling Pathway (198909); BDNF Signaling Pathway (712093); CXCR4-mediated Signaling Events Pathway (137910)

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described. Protein type: Nuclear receptor co-regulator; DNA-binding; Motility/polarity/chemotaxis; Transcription factor. Chromosomal Location of Human Ortholog: 17q21.31. Cellular Component: nucleoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; cytosol; nucleus. Molecular Function: protein dimerization activity; signal transducer activity; ligand-dependent nuclear receptor activity; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; protein phosphatase binding; transcription factor binding; protein kinase binding; glucocorticoid receptor binding; CCR5 chemokine receptor binding. Biological Process: transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; viral reproduction; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; signal transduction; response to estradiol stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; response to drug; nervous system development; intracellular receptor-mediated signaling pathway; eating behavior; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; cell proliferation; response to ethanol; sexual reproduction; positive regulation of transcription from RNA polymerase II promoter; eye photoreceptor cell differentiation; cell motility; phosphorylation. Disease: Hyper-ige Recurrent Infection Syndrome, Autosomal Dominant; Autoimmune Disease, Multisystem, Infantile-onset

15 nmol

365 USD

30 nmol

515