gene knockdown

FTL1 siRNA (Rat)

MBS8234986

15 nmol

365 USD

siRNA

FTL1 siRNA (Rat)

[FTL1]

[FTL; Ferritin light chain 1; Ferritin L subunit 1]

[ferritin light chain 1; Ferritin light chain 1; ferritin light chain 1; ferritin light chain 1; Ferritin L subunit 1]

[FTL1]

[Ftl1; Ftl1; Ftl; Ftl]

FRIL1_RAT

Rat

183

FTL1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

> 97%

Lyophilized powder

Shipped at 4 degree C. Store at -20 degree C for one year.

RNA Interference (RNAi)

Source: Synthetic

Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FTL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

siRNA to inhibit FTL1 expression using RNA interference

84000579

NP_071945.3

NM_022500.4

P02793

20,749 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1262590); Clathrin Derived Vesicle Budding Pathway (1262574); Golgi Associated Vesicle Biogenesis Pathway (1262575); Iron Uptake And Transport Pathway (1262561); Membrane Trafficking Pathway (1262570); Mineral Absorption Pathway (212234); Mineral Absorption Pathway (212220); Relationship Between Glutathione And NADPH Pathway (920874); Scavenging By Class A Receptors Pathway (1262592); Transmembrane Transport Of Small Molecules Pathway (1262516)

iron-storage protein [RGD, Feb 2006]

FTL: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS). It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene. Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3); also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. Belongs to the ferritin family. Protein type: Oxidoreductase. Cellular Component: ferritin complex; membrane; cytoplasm. Molecular Function: identical protein binding; ferric iron binding; iron ion binding. Biological Process: cellular iron ion homeostasis; response to lead ion; iron ion transport

15 nmol

365 USD

30 nmol

515