Anti-TPL2 (pT290) Antibody | MyBioSource MBS8234534 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-TPL2 (pT290) Antibody

catalog :

MBS8234534

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of TPL2 (pT290) expression in HepG2 (A), mouse liver (B), rat liver (C) whole cell lysates.

image 2 :

Immunohistochemical analysis of TPL2 (pT290) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of TPL2 (pT290) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8234534

products type :

Antibody

products full name :

Anti-TPL2 (pT290) Antibody

products short name :

[TPL2]

products name syn :

[COT; ESTF; Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2]

other names :

[mitogen-activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; mitogen-activated protein kinase kinase kinase 8; mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2]

products gene name :

[MAP3K8]

other gene names :

[MAP3K8; MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT; COT; ESTF; TPL-2]

uniprot entry name :

M3K8_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Monkey

sequence length :

467

specificity :

Recognizes endogenous levels of TPL2 (pT290) protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipping: Blue Ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB 1/1500-1/1000. IH 1/100-1/200. IF/IC 1/100/1/500.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TPL2. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to TPL2 (pT290)

ncbi gi num :

346644795

ncbi acc num :

NP_001231063.1

ncbi gb acc num :

NM_001244134.1

uniprot acc num :

P41279

ncbi pathways :

Activated TLR4 Signalling Pathway (1269236); Adaptive Immune System Pathway (1269171); CD28 Co-stimulation Pathway (1269178); CD28 Dependent PI3K/Akt Signaling Pathway (1269179); Costimulation By The CD28 Family Pathway (1269177); Cytokine Signaling In Immune System Pathway (1269310); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Insulin Signaling Pathway (198845); Interleukin-1 Signaling Pathway (1269320)

ncbi summary :

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

uniprot summary :

Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Oncoprotein; EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE-Unique family. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: cytoplasm; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity. Biological Process: activation of MAPKK activity; apoptosis; regulation of cell differentiation; T cell costimulation; MAPKKK cascade; cell cycle; protein amino acid phosphorylation. Disease: Lung Cancer

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage