gene knockdown

GRM8 siRNA (Rat)

MBS8233032

15 nmol

365 USD

siRNA

GRM8 siRNA (Rat)

[GRM8]

[GPRC1H; MGLUR8; Metabotropic glutamate receptor 8; mGluR8]

[metabotropic glutamate receptor 8; Metabotropic glutamate receptor 8; metabotropic glutamate receptor 8; glutamate receptor, metabotropic 8]

[GRM8]

[Grm8; Grm8; Glur8; mGlur; Gprc1h; Mglur8; mGluR8b; Gprc1h; Mglur8; mGluR8]

GRM8_RAT

Synthetic

Rat

908

GRM8 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

> 97%

Lyophilized powder

Shipped at 4 degree C. Store at -20 degree C for one year.

RNA Interference (RNAi)

Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GRM8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

siRNA to inhibit GRM8 expression using RNA interference

11559972

NP_071538.1

NM_022202.1

P70579

101,867 Da

Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway (1262878); G Alpha (i) Signalling Events Pathway (1262881); GPCR Downstream Signaling Pathway (1262879); GPCR Ligand Binding Pathway (1262849); GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway (198511); GPCRs, Other Pathway (198454); Glutamatergic Synapse Pathway (213815); Glutamatergic Synapse Pathway (213811); Neuroactive Ligand-receptor Interaction Pathway (83445); Neuroactive Ligand-receptor Interaction Pathway (462)

metabotropic glutamate receptor that may be involved in synaptic transmission [RGD, Feb 2006]

mGluR8: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Belongs to the G-protein coupled receptor 3 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: GPCR, family 3; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral. Cellular Component: presynaptic membrane; postsynaptic membrane; neuron projection; cell soma; integral to plasma membrane; integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; group III metabotropic glutamate receptor activity; glutamate receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; synaptic transmission, glutamatergic; detection of mechanical stimulus involved in sensory perception of pain; negative regulation of cAMP biosynthetic process; regulation of synaptic transmission, glutamatergic; sensory perception of smell; regulation of neurotransmitter secretion; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway; sensory perception of pain

15 nmol

365 USD

30 nmol

515