gene knockdown

FOXRED1 siRNA (Human)

MBS8232003

15 nmol

365 USD

siRNA

FOXRED1 siRNA (Human)

[FOXRED1]

[FAD-dependent oxidoreductase domain-containing protein 1]

[FAD-dependent oxidoreductase domain-containing protein 1; FAD-dependent oxidoreductase domain-containing protein 1; FAD-dependent oxidoreductase domain-containing protein 1; FAD-dependent oxidoreductase domain containing 1]

[FOXRED1]

[FOXRED1; FOXRED1; H17; FP634]

FXRD1_HUMAN

Human

486

FOXRED1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

> 97%

Lyophilized powder

Shipped at 4 degree C. Store at -20 degree C for one year.

RNA Interference (RNAi)

Source: Synthetic. Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FOXRED1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo. Directions for use: We recommend transfection with 100nM siRNA 48 to 72 hours prior to cell lysis.Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20pmol for each well.).

siRNA to inhibit FOXRED1 expression using RNA interference

8923708

NP_060017.1

NM_017547.3

Q96CU9

This gene encodes a protein that contains a FAD-dependent oxidoreductase domain. The encoded protein is localized to the mitochondria and may function as a chaperone protein required for the function of mitochondrial complex I. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]

FOXRED1: Defects in FOXRED1 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical manifestations from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q24.2. Cellular Component: mitochondrion; integral to membrane. Molecular Function: oxidoreductase activity. Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency

15 nmol

365 USD

30 nmol

515