NR0B1 siRNA (Mouse) | MyBioSource MBS8231279 product information

product summary

company name :

MyBioSource

product type :

gene knockdown

product name :

NR0B1 siRNA (Mouse)

catalog :

MBS8231279

quantity :

15 nmol

price :

365 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS8231279

products type :

siRNA

products full name :

NR0B1 siRNA (Mouse)

products short name :

[NR0B1]

products name syn :

[AHCH; DAX1; Nuclear receptor subfamily 0 group B member 1; Nuclear receptor DAX-1]

other names :

[nuclear receptor subfamily 0 group B member 1; Nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0, group B, member 1; Nuclear receptor DAX-1]

products gene name :

[NR0B1]

other gene names :

[Nr0b1; Nr0b1; AHX; Ahc; Ahch; Dax1; DAX-1; Ahch; Dax1]

uniprot entry name :

NR0B1_MOUSE

reactivity :

Mouse

sequence length :

472

specificity :

NR0B1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

purity :

> 97%

form :

Lyophilized powder

storage stability :

Shipped at 4 degree C. Store at -20 degree C for one year.

tested application :

RNA Interference (RNAi)

other info1 :

Source: Synthetic

other info2 :

Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommend transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NR0B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

products description :

siRNA to inhibit NR0B1 expression using RNA interference

ncbi gi num :

6671531

ncbi acc num :

NP_031456.1

ncbi gb acc num :

NM_007430.5

uniprot acc num :

Q61066

ncbi pathways :

Androgen Receptor Signaling Pathway (198319); Gene Expression Pathway (1253646); Generic Transcription Pathway (1253647); Nuclear Receptor Transcription Pathway (1253649); Nuclear Receptors Pathway (198318); PluriNetWork Pathway (198307)

ncbi summary :

This gene encodes an orphan nuclear receptor protein that plays a key role in differentiation of the gonads. This protein regulates steroidogenic factor 1 (Sf-1) in a dose-dependent manner, sometimes functioning as a repressor of SF-1 target genes, and sometimes functioning as a co-activator. Overexpression of this gene can cause feminization of the XY male gonads. This gene is also involved in the maintenance of embryonic stem cell pluripotancy. Mutations in the related gene in human cause congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, May 2015]

uniprot summary :

NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described. Protein type: Nuclear receptor. Cellular Component: polysomal ribosome; membrane; cytoplasm; nucleus. Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; RNA binding; transcription factor binding; steroid hormone receptor binding; DNA hairpin binding; protein binding; DNA binding; AF-2 domain binding; sequence-specific DNA binding; double-stranded DNA binding; steroid hormone receptor activity; transcription factor activity; transcription corepressor activity. Biological Process: transcription from RNA polymerase II promoter; negative regulation of steroid hormone receptor signaling pathway; intracellular receptor-mediated signaling pathway; gonad development; transcription, DNA-dependent; adrenal gland development; negative regulation of transcription factor activity; male gonad development; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; sex determination; negative regulation of cell differentiation; protein localization; Leydig cell differentiation; regulation of transcription, DNA-dependent; male sex determination; spermatogenesis; steroid hormone mediated signaling; cell differentiation; negative regulation of transcription, DNA-dependent; steroid biosynthetic process

size1 :

15 nmol

price1 :

365 USD

size2 :

30 nmol

price2 :

515

more info or order :

MyBioSource product webpage