gene knockdown

GSTA1 siRNA (Rat)

MBS8230272

15 nmol

365 USD

siRNA

GSTA1 siRNA (Rat)

[GSTA1]

[Glutathione S-transferase alpha-1; GST 1-1; GST 1a-1a; GST A1-1; GST B; Glutathione S-transferase Ya-1; GST Ya1; Ligandin]

[glutathione S-transferase alpha-1; Glutathione S-transferase alpha-1; glutathione S-transferase alpha-1; glutathione S-transferase alpha 2; GST 1-1; GST 1a-1a; GST A1-1; GST B; Glutathione S-transferase Ya-1; GST Ya1]

[GSTA1]

[Gsta2; Gsta1; Gsta1; GST Ya1]

GSTA1_RAT

Synthetic

Rat

222

GSTA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

> 97%

Lyophilized powder

Ships with blue ice. Store at -20 degree C for one year.

RNA Interference (RNAi)

Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommend transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GSTA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

siRNA to inhibit GSTA1 expression using RNA interference

51036637

NP_058709.2

NM_017013.4

P00502

Biological Oxidations Pathway (1263416); Chemical Carcinogenesis Pathway (673233); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83424); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (1263440); Glutathione Metabolism Pathway (83365); Glutathione Metabolism Pathway (198431); Glutathione Metabolism Pathway (343); Keap1-Nrf2 Pathway (219759)

mRNA expression is induced by phenobarbital and by trans-stilbene oxide [RGD, Feb 2006]

GSTA5: Belongs to the GST superfamily. Alpha family. Homodimer. Protein type: Nuclear envelope; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Transferase; EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - metabolism by cytochrome P450. Cellular Component: nuclear outer membrane; nucleus; cytosol. Molecular Function: glutathione binding; glutathione transferase activity; drug binding. Biological Process: metabolic process; xenobiotic catabolic process; aging

15 nmol

365 USD

30 nmol

515