Anti-HLA-DQB1 Antibody | MyBioSource MBS8229850 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-HLA-DQB1 Antibody

catalog :

MBS8229850

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of HLA-DQB1 expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates.

product information

catalog number :

MBS8229850

products type :

Antibody

products full name :

Anti-HLA-DQB1 Antibody

products short name :

[HLA-DQB1]

products name syn :

[HLA-DQB1; HLA-DQB; HLA class II histocompatibility antigen, DQ beta 1 chain; MHC class II antigen DQB1; HLA-DQB2; HLA-DXB; HLA class II histocompatibility antigen, DQ beta 2 chain; HLA class II histocompatibility antigen, DX beta chain; MHC class II antigen DQB2]

other names :

[HLA class II histocompatibility antigen, DQ beta 1 chain isoform 1; HLA class II histocompatibility antigen, DQ beta 1 chain; HLA class II histocompatibility antigen, DQ beta 1 chain; major histocompatibility complex, class II, DQ beta 1; MHC class II antigen DQB1]

products gene name :

[HLA-DQB1]

products gene name syn :

[HLA-DQB2]

other gene names :

[HLA-DQB1; HLA-DQB1; IDDM1; CELIAC1; HLA-DQB; HLA-DQB]

uniprot entry name :

DQB1_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat, Bovine, Monkey, Sheep

sequence length :

261

specificity :

Recognizes endogenous levels of HLA-DQB1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB)

app notes :

WB (1/500 - 1/1000)

image1 heading :

Western Blot (WB)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HLA-DQB1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to HLA-DQB1

ncbi gi num :

345461079

ncbi acc num :

NP_001230891.1

ncbi gb acc num :

NM_001243962.1

uniprot acc num :

P01920

ncbi mol weight :

29,991 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

ncbi summary :

HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

uniprot summary :

HLA-DQB1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane; endosome membrane; MHC class II protein complex. Molecular Function: MHC class II receptor activity; peptide antigen binding. Biological Process: humoral immune response mediated by circulating immunoglobulin; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; immunoglobulin production during immune response; T cell receptor signaling pathway. Disease: Celiac Disease; Creutzfeldt-jakob Disease; Multiple Sclerosis, Susceptibility To

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage