THRB siRNA (Human) | MyBioSource MBS8229008 product information

product summary

company name :

MyBioSource

product type :

gene knockdown

product name :

THRB siRNA (Human)

catalog :

MBS8229008

quantity :

15 nmol

price :

365 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS8229008

products type :

siRNA

products full name :

THRB siRNA (Human)

products short name :

[THRB]

products name syn :

[ERBA2; NR1A2; THR1; Thyroid hormone receptor beta; Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta]

other names :

[thyroid hormone receptor beta; Thyroid hormone receptor beta; thyroid hormone receptor beta; thyroid hormone receptor, beta; Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta]

products gene name :

[THRB]

other gene names :

[THRB; THRB; GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA; ERBA2; NR1A2; THR1]

uniprot entry name :

THB_HUMAN

reactivity :

Human

sequence length :

461

specificity :

THRB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

purity :

> 97%

form :

Lyophilized powder

storage stability :

Shipped at 4 degree C. Store at -20 degree C for one year.

tested application :

RNA Interference (RNAi)

other info1 :

Source: Synthetic

other info2 :

Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human THRB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

products description :

siRNA to inhibit THRB expression using RNA interference

ncbi gi num :

40806162

ncbi acc num :

NP_000452.2

ncbi gb acc num :

NM_000461.4

uniprot acc num :

P10828

ncbi mol weight :

54,449 Da

ncbi pathways :

Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (1269652); Nuclear Receptors Pathway (198848); SIDS Susceptibility Pathways (198901); Thyroid Hormone Signaling Pathway (946598)

ncbi summary :

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TR-beta1: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Homolog of the viral oncogene erbB. Two splice variant isoforms have been described. Protein type: Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 3p24.2. Cellular Component: nucleoplasm; nuclear chromatin; nucleus. Molecular Function: protein binding; enzyme binding; DNA binding; zinc ion binding; sequence-specific DNA binding; chromatin DNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity; transcription corepressor activity. Biological Process: negative regulation of female receptivity; transcription initiation from RNA polymerase II promoter; organ morphogenesis; sensory perception of sound; female courtship behavior; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction. Disease: Thyroid Hormone Resistance, Selective Pituitary; Thyroid Hormone Resistance, Generalized, Autosomal Dominant; Thyroid Hormone Resistance, Generalized, Autosomal Recessive

size1 :

15 nmol

price1 :

365 USD

size2 :

30 nmol

price2 :

515

more info or order :

MyBioSource product webpage