gene knockdown

NCR3LG1 siRNA (Human)

MBS8225935

15 nmol

365 USD

siRNA

NCR3LG1 siRNA (Human)

[NCR3LG1]

[B7H6; Natural cytotoxicity triggering receptor 3 ligand 1; B7 homolog 6; B7-H6]

[natural cytotoxicity triggering receptor 3 ligand 1; Natural cytotoxicity triggering receptor 3 ligand 1; natural cytotoxicity triggering receptor 3 ligand 1; natural killer cell cytotoxicity receptor 3 ligand 1; B7 homolog 6; B7-H6]

[NCR3LG1]

[NCR3LG1; NCR3LG1; B7H6; B7-H6; DKFZp686O24166; B7H6; B7-H6]

NR3L1_HUMAN

Human

454

NCR3LG1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

> 97%

Lyophilized powder

Shipped at 4 degree C. Store at -20 degree C for one year.

RNA Interference (RNAi)

Source: Synthetic

Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NCR3LG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

siRNA to inhibit NCR3LG1 expression using RNA interference

320461732

NP_001189368.1

NM_001202439.2

Q68D85

Adaptive Immune System Pathway (1269171); Immune System Pathway (1269170); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1269201)

B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]

B7-H6: Triggers NCR3-dependent natural killer cell activation. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 11p15.1. Cellular Component: plasma membrane; integral to membrane; viral capsid. Molecular Function: structural molecule activity

15 nmol

365 USD

30 nmol

515