Anti-Phospholamban (pS16/T17) Antibody | MyBioSource MBS8224597 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospholamban (pS16/T17) Antibody

catalog :

MBS8224597

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Phospholamban (pS16/T17) expression in human heart (A) whole cell lysates.

image 2 :

Immunofluorescent analysis of Phospholamban (pS16/T17) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS8224597

products type :

Antibody

products full name :

Anti-Phospholamban (pS16/T17) Antibody

products short name :

[Phospholamban]

products name syn :

[PLB; Cardiac phospholamban; PLB]

other names :

[cardiac phospholamban; Cardiac phospholamban; cardiac phospholamban; phospholamban]

products gene name :

[PLN]

other gene names :

[PLN; PLN; PLB; CMD1P; CMH18; PLB; PLB]

uniprot entry name :

PPLA_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat, Bovine, Dog, Porcine, Rabbit

sequence length :

specificity :

Recognizes endogenous levels of Phospholamban (pS16/T17) protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

WB, IF/IC

app notes :

WB (1/500 - 1/1000). IF/IC (1/100-1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Phospholamban (pS16/T17). The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Phospholamban (pS16/T17)

ncbi gi num :

4505887

ncbi acc num :

NP_002658.1

ncbi gb acc num :

NM_002667.3

uniprot acc num :

P26678

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Integrated Pancreatic Cancer Pathway (711360); Ion Channel Transport Pathway (1269950); Ion Transport By P-type ATPases Pathway (1269951)

ncbi summary :

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]

uniprot summary :

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. Protein type: Inhibitor; Membrane protein, integral. Chromosomal Location of Human Ortholog: 6q22.1. Cellular Component: sarcoplasmic reticulum membrane; mitochondrion; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; mitochondrial membrane; vesicle. Molecular Function: enzyme inhibitor activity; identical protein binding; protein binding; ATPase inhibitor activity; calcium channel regulator activity; ATPase binding. Biological Process: cardiac muscle development; negative regulation of calcium ion transport; cytosolic calcium ion homeostasis; negative regulation of heart rate; blood circulation; response to testosterone stimulus; regulation of calcium ion transport; regulation of heart contraction; response to insulin stimulus; response to zinc ion; calcium ion transport; negative regulation of catalytic activity; negative regulation of ATPase activity; protein homooligomerization; regulation of the force of heart contraction. Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage