gene knockdown

ARF5 siRNA (Human)

MBS8223552

15 nmol

365 USD

siRNA

ARF5 siRNA (Human)

[ARF5]

[ADP-ribosylation factor 5]

[ADP-ribosylation factor 5; ADP-ribosylation factor 5; ADP-ribosylation factor 5; ADP-ribosylation factor 5]

[ARF5]

[ARF5; ARF5]

ARF5_HUMAN

Human

180

ARF5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

> 97%

Lyophilized powder

Shipped at 4 degree C. Store at -20 degree C for one year.

RNA Interference (RNAi)

Source: Synthetic. Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well). Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARF5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

siRNA to inhibit ARF5 expression using RNA interference

4502209

NP_001653.1

NM_001662.3

P84085

20,530 Da

Class I PI3K Signaling Events Pathway (138022); Endocytosis Pathway (102279); Endocytosis Pathway (102181)

This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]

ARF5: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family. Protein type: G protein, monomeric; G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7q31.3. Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; plasma membrane. Molecular Function: GTPase activity; protein binding; GTP binding. Biological Process: vesicle-mediated transport; protein transport; metabolic process; small GTPase mediated signal transduction

15 nmol

365 USD

30 nmol

515