Alkaline Phosphatase Assay Kit | MyBioSource MBS822355 product information

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product summary

company name :

MyBioSource

product type :

other

product name :

Alkaline Phosphatase Assay Kit

catalog :

MBS822355

quantity :

100 Assays

price :

215 USD

product information

catalog number :

MBS822355

products type :

Assay Kit

products full name :

Alkaline Phosphatase Assay Kit

products short name :

[Alkaline Phosphatase]

other names :

[alkaline phosphatase; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme]

other gene names :

[ALPL; ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP; AP-TNAP; TNSALP]

uniprot entry name :

PPBT_HUMAN

reactivity :

General

sequence length :

524

storage stability :

Shipped and store at 4 degree C for 6 months.

tested application :

Functional Assay

other info1 :

Samples: Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples.

products description :

Background/Introduction: Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. The assay is initiated with the enzymatic hydrolysis of the disodium phenyl phosphate by acid phosphatase. The enzyme catalysed reaction products can be measured at a colorimetric readout at 510 nm.

ncbi gi num :

178462

ncbi acc num :

AAB59378.1

ncbi mol weight :

51,045 Da

ncbi pathways :

AGE/RAGE Pathway (698754); BDNF Signaling Pathway (712093); Endochondral Ossification Pathway (198812); Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Metabolic Pathways (132956); TNF-alpha/NF-kB Signaling Pathway (198884)

ncbi summary :

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

uniprot summary :

ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; Motility/polarity/chemotaxis; EC 3.1.3.1; Membrane protein, GPI anchor; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 1p36.12. Cellular Component: extracellular matrix; extracellular space; membrane; integral to membrane; plasma membrane. Molecular Function: protein binding; pyrophosphatase activity; alkaline phosphatase activity; metal ion binding. Biological Process: response to antibiotic; osteoblast differentiation; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification. Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood

size1 :

100 Assays

price1 :

215 USD