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Trehalase Assay Kit

MBS822353

100 Assays

370 USD

Assay Kit

Trehalase Assay Kit

[Trehalase]

[trehalase isoform 1; Trehalase; trehalase; alpha,alpha-trehalose glucohydrolase; trehalase (brush-border membrane glycoprotein); Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase]

[TREH; TREH; TRE; TREA; TREA]

TREA_HUMAN

General

583

Shipped and store at 4 degree C for 6 months.

Functional Assay

Typical Testing Data/Standard Curve (for reference only)

Background/Introduction: Transglutaminases (EC 2.3.2.13) are calcium dependent enzymes that catalyze the post-translational modification of proteins by formation of isopeptide bonds. This occurs either through protein cross-linking via formation of gamma-glutamyl-epsilon-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The transglutaminase enzyme family comprises the intracellular forms (TG1, TG3 and TG5) expressed mostly in the epithelial tissue; TG2 which is both intracellular and extracellular and expressed in various tissue types; TG4 which is expressed in the prostate gland; factor XIII which is expressed in blood; TG6 and TG7, whose tissue distribution is unknown and band 4.2 (lacking enzymatic activity) which is present on erythrocyte membranes. Transglutaminases also exhibit GTPase, phosphodiesterase and protein kinase activity. Transglutaminases are associated with certain neurological and autoimmune disorders and also cancer. MBS, Inc. Transglutaminase Activity Assay kit utilizes the deamidation reaction of the transglutaminase enzyme with a donor and acceptor substrate resulting in the formation of a hydroxamate product. The hydroxamate product reacts with the Stop Solution forming a purple complex that can be measured colorimetrically at 525 nm. The limit of quantification of this assay is ~10 uU or 80 ng of recombinant hTG2 enzyme.

116284412

NP_009111.2

NM_007180.2

62,941 Da

Digestion Of Dietary Carbohydrate Pathway (106197); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196); Myoclonic Epilepsy Of Lafora Pathway (980469); Starch And Sucrose Metabolism Pathway (82974); Starch And Sucrose Metabolism Pathway (344); Trehalose Degradation II (trehalase) Pathway (142404)

This gene encodes an enzyme that hydrolyses trehalose, a disaccharide formed from two glucose molecules found mainly in fungi, plants, and insects. A partial duplication of this gene is located adjacent to this locus on chromosome 11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

TREH: Intestinal trehalase is probably involved in the hydrolysis of ingested trehalose. Deficiency of TREH results in isolated trehalose intolerance that causes gastrointestinal symptoms after ingestion of edible mushrooms. Belongs to the glycosyl hydrolase 37 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.2.1.28; Membrane protein, GPI anchor; Carbohydrate Metabolism - starch and sucrose; Hydrolase. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: anchored to plasma membrane; plasma membrane. Molecular Function: alpha,alpha-trehalase activity. Biological Process: polysaccharide digestion; organ morphogenesis; trehalose catabolic process; trehalose metabolic process; carbohydrate metabolic process; pathogenesis. Disease: Trehalase Deficiency

100 Assays

370 USD