catalog number :
MBS822350
products type :
Assay Kit
products full name :
Nitrate Reductase Assay Kit
products short name :
[Nitrate Reductase]
other names :
[nitrate reductase; Nitrate reductase; nitrate reductase; Nitrate reductase]
other gene names :
[Marky_1807; Marky_1807]
uniprot entry name :
F2NPN8_MARHT
storage stability :
Shipped and store at 4 degree C for 6 months.
tested application :
Functional Assay
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Various Biological Samples/Preparations
products description :
Background/Introduction: Neprilysin (NEP, EC 3.4.24.11), also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid beta (Abeta) peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, and Alzheimer's disease. NEP has also been used as a biological marker of a type of child leukemia and the detection of NEP in endometrial stromal cells had been proposed as a helpful tool in diagnosis of endometriosis. NEP is currently a focus of major interest in cardiovascular and neurological research. Neprilysin Activity Kit utilizes the ability of an active NEP to cleave a synthetic substrate (Abz-based peptide) to release a free fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. The substrate is specific to NEP and can differentiate the NEP activity from Trypsin and other structurally similar zinc metalloproteinase in biological samples such as Angiotensin-Converting Enzymes (ACE1, ACE2), Endothelin Converting Enzymes (ECE1, ECE2). Our assay kit is simple, specific and can detect as low as 20 uU/mg of NEP activity.
ncbi acc num :
YP_004368649.1
ncbi gb acc num :
NC_015387.1
ncbi mol weight :
106,991 Da
ncbi pathways :
Microbial Metabolism In Diverse Environments Pathway (216627); Nitrogen Metabolism Pathway (216623); Nitrogen Metabolism Pathway (416)