Anti-Aggrecan Antibody | MyBioSource MBS822299 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Aggrecan Antibody

catalog :

MBS822299

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Aggrecan expression in HeLa colchicine-treated (A), mouse kidney (B), PC12 colchicine-treated (C), NIH3T3 (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of Aggrecan staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

product information

catalog number :

MBS822299

products type :

Antibody

products full name :

Anti-Aggrecan Antibody

products short name :

[Aggrecan]

products name syn :

[AGC1; CSPG1; MSK16; Aggrecan core protein; Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1; Chondroitin sulfate proteoglycan 1]

other names :

[Aggrecan core protein; Aggrecan core protein; aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1; aggrecan; Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1; Chondroitin sulfate proteoglycan 1]

products gene name :

[ACAN]

other gene names :

[ACAN; ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1]

uniprot entry name :

PGCA_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep

sequence length :

2415

specificity :

Recognizes endogenous levels of Aggrecan protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Ships on blue ice. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB (1/500 - 1/1000), IHC (1/100 - 1/200)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Aggrecan. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Aggrecan

ncbi gi num :

129886

ncbi acc num :

P16112.2

uniprot acc num :

P16112

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (576263); Disease Pathway (530764); ECM Proteoglycans Pathway (833812); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Metabolism Pathway (645297); Keratan Sulfate Biosynthesis Pathway (645302); Keratan Sulfate Degradation Pathway (645303); Keratan Sulfate/keratin Metabolism Pathway (645301)

ncbi summary :

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Secreted, signal peptide; Secreted; Cell adhesion. Chromosomal Location of Human Ortholog: 15q26.1. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region. Molecular Function: protein binding; metal ion binding; extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding. Biological Process: keratan sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; pathogenesis; keratan sulfate catabolic process; proteolysis; cell adhesion; skeletal development. Disease: Spondyloepiphyseal Dysplasia, Kimberley Type; Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage