product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-MARK Antibody
catalog :
MBS8221493
quantity :
0.03 mL
price :
155 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of MARK expression in A549 (A), HeLa (B) whole cell lysates.
image 2 :
Immunohistochemical analysis of MARK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
image 3 :
Immunofluorescent analysis of MARK staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
product information
catalog number :
MBS8221493
products type :
Antibody
products full name :
Anti-MARK Antibody
products short name :
[MARK]
products name syn :
[MARK1; KIAA1477; MARK; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c; MARK2; EMK1; Serine/threonine-protein kinase MARK2; ELKL motif kinase 1; EMK-1; MAP/microtubule affinity-regulating]
other names :
[serine/threonine-protein kinase MARK1 isoform 4; Serine/threonine-protein kinase MARK1; serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c]
products gene name :
[MARK1]
products gene name syn :
[MARK2; MARK3; MARK4]
other gene names :
[MARK1; MARK1; MARK; Par1c; Par-1c; Par-1c; Par1c]
uniprot entry name :
MARK1_HUMAN
clonality :
Polyclonal
host :
Rabbit
reactivity :
Human, Mouse, Rat, Zebrafish
sequence length :
758
specificity :
Recognizes endogenous levels of MARK protein.
purity :
The antibody was purified by immunogen affinity chromatography.
form :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
storage stability :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
tested application :
Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
app notes :
WB (1/500 - 1/1000). IHC (1/100 - 1/200). IF/ICC (1/100 - 1/500)
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Immunofluorescence (IF)
other info1 :
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MARK. The exact sequence is proprietary.
other info2 :
Gene Symbol: MARK1
products description :
Rabbit polyclonal antibody to MARK
ncbi gi num :
554790379
ncbi acc num :
NP_001273057.1
ncbi gb acc num :
NM_001286128.1
uniprot acc num :
Q9P0L2
ncbi pathways :
Regulation Of Microtubule Cytoskeleton Pathway (755439)
uniprot summary :
MARK1: a member of the CAMKL family of protein kinases. The MARK group of kinases is apparently involved in the control of cell polarity, microtubule stability and cancer. MARK1 forms a tight association with Tau in neurofibrillary tangles in Alzheimer disease tissue. Protein type: Protein kinase, CAMK; EC 2.7.11.1; EC 2.7.11.26; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; MARK subfamily. Chromosomal Location of Human Ortholog: 1q41. Cellular Component: microtubule cytoskeleton; cytoskeleton; cytoplasm; plasma membrane. Molecular Function: protein serine/threonine kinase activity; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylserine binding; tau-protein kinase activity; magnesium ion binding; ATP binding. Biological Process: Wnt receptor signaling pathway; neuron migration; cytoskeleton organization and biogenesis; protein amino acid phosphorylation
size1 :
0.03 mL
price1 :
155 USD
size2 :
0.1 mL
price2 :
220
size3 :
0.2 mL
price3 :
295
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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