Anti-Cytochrome P450 26A1 Antibody | MyBioSource MBS822084 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Cytochrome P450 26A1 Antibody

catalog :

MBS822084

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Cytochrome P450 26A1 expression in HepG2 (A), HEK293T (B) whole cell lysates.

image 2 :

Immunofluorescent analysis of Cytochrome P450 26A1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS822084

products type :

Antibody

products full name :

Anti-Cytochrome P450 26A1 Antibody

products short name :

[Cytochrome P450 26A1]

products name syn :

[CYP26; P450RAI1; Cytochrome P450 26A1; Cytochrome P450 retinoic acid-inactivating 1; Cytochrome P450RAI; hP450RAI; Retinoic acid 4-hydroxylase; Retinoic acid-metabolizing cytochrome]

other names :

[cytochrome P450 26A1 isoform 1; Cytochrome P450 26A1; cytochrome P450 26A1; hP450RAI; cytochrome P450RAI; retinoic acid 4-hydroxylase; P450, retinoic acid-inactivating, 1; retinoic acid-metabolizing cytochrome; cytochrome P450 retinoic acid-inactivating 1; cytochrome P450, subfamily XXVIA, polypeptide 1; cytochrome P450, family 26, subfamily A, polypeptide 1; Cytochrome P450 retinoic acid-inactivating 1; Cytochrome P450RAI; hP450RAI; Retinoic acid 4-hydroxylase; Retinoic acid-metabolizing cytochrome]

products gene name :

[CYP26A1]

other gene names :

[CYP26A1; CYP26A1; CP26; CYP26; P450RAI; P450RAI1; CYP26; P450RAI1; Cytochrome P450RAI; hP450RAI]

uniprot entry name :

CP26A_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Pig

sequence length :

497

specificity :

Recognizes endogenous levels of Cytochrome P450 26A1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipping: Blue Ice. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC), Immunoprecipitation (IP)

app notes :

WB (1/500 - 1/1000). IF/ICC (1/100 - 1/500). IP (1/10 - 1/100)

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info2 :

Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 26A1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Cytochrome P450 26A1

ncbi gi num :

16933530

ncbi acc num :

NP_000774.2

ncbi gb acc num :

NM_000783.3

uniprot acc num :

O43174

ncbi pathways :

Adipogenesis Pathway (198832); Biological Oxidations Pathway (105698); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Metabolic Pathways (132956); Metabolism Pathway (477135); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887); Phase 1 - Functionalization Of Compounds Pathway (105699); Retinol Metabolism Pathway (83020); Retinol Metabolism Pathway (406); Vitamin A And Carotenoid Metabolism Pathway (920901)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

uniprot summary :

CYP26A1: Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18- hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA. Belongs to the cytochrome P450 family. Protein type: Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; EC 1.14.-.-; Oxidoreductase. Chromosomal Location of Human Ortholog: 10q23-q24. Cellular Component: endoplasmic reticulum membrane. Molecular Function: retinoic acid binding; iron ion binding; retinoic acid 4-hydroxylase activity; heme binding; oxygen binding. Biological Process: retinoic acid receptor signaling pathway; anterior/posterior pattern formation; negative regulation of retinoic acid receptor signaling pathway; vitamin metabolic process; central nervous system development; neural crest cell development; metabolic process; xenobiotic metabolic process

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage