Anti-GADD153 Antibody | MyBioSource MBS822080 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-GADD153 Antibody

catalog :

MBS822080

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of GADD153 expression in HeLa (A), MCF7 (B), SP2/0 (C), PC12 (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of GADD153 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of GADD153 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS822080

products type :

Antibody

products full name :

Anti-GADD153 Antibody

products short name :

[GADD153]

products name syn :

[CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153]

other names :

[DNA damage-inducible transcript 3 protein isoform 1; DNA damage-inducible transcript 3 protein; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153; DNA-damage-inducible transcript 3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153]

products gene name :

[DDIT3]

other gene names :

[DDIT3; DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10]

uniprot entry name :

DDIT3_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat, Bovine, Pig, Sheep

sequence length :

192

specificity :

Recognizes endogenous levels of GADD153 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IHC (1/100-1/200), IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GADD153. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to GADD153

ncbi gi num :

304282225

ncbi acc num :

NP_001181982.1

ncbi gb acc num :

NM_001195053.1

uniprot acc num :

P35638

ncbi mol weight :

21,700 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); ATF4 Activates Genes Pathway (530772); ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Adipogenesis Pathway (198832); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Metabolism Of Proteins Pathway (106230); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188)

ncbi summary :

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

uniprot summary :

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform. Protein type: Autophagy; DNA-binding; Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 12q13.1-q13.2. Cellular Component: nucleoplasm; late endosome; cytosol; nucleus. Molecular Function: protein binding; leucine zipper domain binding; DNA binding; protein heterodimerization activity; transcription corepressor activity; transcription factor activity; transcription factor binding. Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; unfolded protein response; negative regulation of transcription factor activity; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; response to starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of myoblast differentiation; response to DNA damage stimulus. Disease: Myxoid Liposarcoma

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage