Anti-ETS1 (pT38) Antibody | MyBioSource MBS822058 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-ETS1 (pT38) Antibody

catalog :

MBS822058

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of ETS1 (pT38) expression in LO2 (A), Jurkat (B), HepG2 (C), NIH3T3 (D), rat liver (E) whole cell lysates.

image 2 :

Immunohistochemical analysis of ETS1 (pT38) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of ETS1 (pT38) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS822058

products type :

Antibody

products full name :

Anti-ETS1 (pT38) Antibody

products short name :

[ETS1 (pT38)]

products name syn :

[EWSR2; Protein C-ets-1; p54]

other names :

[protein C-ets-1 isoform 1; Protein C-ets-1; protein C-ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; v-ets avian erythroblastosis virus E26 oncogene homolog 1; p54]

products gene name :

[ETS1]

other gene names :

[ETS1; ETS1; p54; ETS-1; EWSR2; EWSR2]

uniprot entry name :

ETS1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Chicken, Dog, Pig, Rabbit

sequence length :

485

specificity :

Recognizes endogenous levels of ETS1 (pT38) protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Entrez Gene: 2113 (Human); 23871 (Mouse); 24356 (Rat). SwissProt: P14921 (Human); P27577 (Mouse); P41156 (Rat)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ETS1. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to ETS1 (pT38)

ncbi gi num :

219689118

ncbi acc num :

NP_001137292.1

ncbi gb acc num :

NM_001143820.1

uniprot acc num :

P14921

ncbi mol weight :

30,860 Da

ncbi pathways :

Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); BCR Signaling Pathway (138058); C-MYB Transcription Factor Network Pathway (138073); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Dorso-ventral Axis Formation Pathway (114227); Dorso-ventral Axis Formation Pathway (472); HIF-1-alpha Transcription Factor Network Pathway (138045); HIF-2-alpha Transcription Factor Network Pathway (137956); HTLV-I Infection Pathway (373901)

ncbi summary :

This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

uniprot summary :

Ets-1: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus oncogene. The DNA binding activity of Ets1 is controlled by kinases and transcription factors. It contributes to the regulation of cellular differentiation in hematopoietic cells. Ets1 promotes invasive behavior in endothelial cells, vascular smooth muscle cells and epithelial cancer cells. Regulates the expression of MMP1, MMP3, MMP9 and uPA as well as of VEGF and VEGFR gene expression. Two alternatively spliced isoforms have been described. Protein type: Motility/polarity/chemotaxis; Transcription factor; Oncoprotein; DNA-binding. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: nucleoplasm; transcription factor complex; intercellular bridge; cytoplasm; nucleus. Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; hypothalamus development; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; cell motility involved in cell locomotion; PML body organization and biogenesis; female pregnancy; positive regulation of cell motility; angiogenesis involved in wound healing; regulation of angiogenesis; response to estradiol stimulus; negative regulation of cell cycle; regulation of apoptosis; response to antibiotic; negative regulation of cell proliferation; positive regulation of angiogenesis; pituitary gland development; response to mechanical stimulus; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; immune response

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage