Anti-SMAD2 (pS467) Antibody | MyBioSource MBS8220276 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-SMAD2 (pS467) Antibody

catalog :

MBS8220276

quantity :

0.03 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, cow, zebrafish

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of SP1 (pT453) expression in Jurkat (A), mouse melanoma cancer (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of SP1 (pT453) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of SP1 (pT453) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

product information

catalog number :

MBS8220276

products type :

Antibody

products full name :

Anti-SMAD2 (pS467) Antibody

products short name :

[SMAD2]

products name syn :

[MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2]

other names :

[mothers against decapentaplegic homolog 2 isoform 1; Mothers against decapentaplegic homolog 2; mothers against decapentaplegic homolog 2; SMAD family member 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2]

products gene name :

[SMAD2]

other gene names :

[SMAD2; SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2]

uniprot entry name :

SMAD2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rabbit, Bovine, Chicken, Monkey, Sheep, Zebrafish, Rat, Pig

sequence length :

467

specificity :

Recognizes endogenous levels of SMAD2 (pS467) protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresences (IF), Immunocytochemistry (ICC)

app notes :

ELISA (1/5000 - 1/10000). WB (1/500 - 1/1000). IHC (1/100 - 1/200). IF (1/100 - 1/500). ICC (1/100 - 1/500)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SMAD2 (pS467). The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to SMAD2 (pS467)

ncbi gi num :

51173730

ncbi acc num :

NP_001003652.1

ncbi gb acc num :

NM_001003652.3

uniprot acc num :

Q15796

ncbi pathways :

AGE/RAGE Pathway (698754); Activin Signaling Pathway (1084757); Activin Signaling Pathway (1108220); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Alpha6-Beta4 Integrin Signaling Pathway (198807); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795)

ncbi summary :

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

uniprot summary :

SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 18q21.1. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; cytosol; nucleus. Molecular Function: transcription activator binding; metal ion binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transcription factor activity; transforming growth factor beta receptor binding. Biological Process: developmental growth; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; palate development; negative regulation of transcription from RNA polymerase II promoter; primary microRNA processing; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; pancreas development; response to glucose stimulus; positive regulation of BMP signaling pathway; pericardium development; transcription initiation from RNA polymerase II promoter; cell fate commitment; transcription, DNA-dependent; regulation of binding; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; endoderm formation; insulin secretion; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; lung development

size1 :

0.03 mL

price1 :

165 USD

size2 :

0.1 mL

price2 :

245

size3 :

0.2 mL

price3 :

350

more info or order :

MyBioSource product webpage