Anti-Cytochrome P450 2J2 Antibody | MyBioSource MBS822016 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Cytochrome P450 2J2 Antibody

catalog :

MBS822016

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Cytochrome P450 2J2 expression in HepG2 (A), A549 (B) whole cell lysates.

image 2 :

Immunohistochemical analysis of Cytochrome P450 2J2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of Cytochrome P450 2J2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

catalog number :

MBS822016

products type :

Antibody

products full name :

Anti-Cytochrome P450 2J2 Antibody

products short name :

[Cytochrome P450 2J2]

products name syn :

[Cytochrome P450 2J2; Arachidonic acid epoxygenase; CYPIIJ2]

other names :

[cytochrome P450 2J2; Cytochrome P450 2J2; cytochrome P450 2J2; CYPIIJ2; microsomal monooxygenase; arachidonic acid epoxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2; cytochrome P450, family 2, subfamily J, polypeptide 2; Arachidonic acid epoxygenase; CYPIIJ2]

products gene name :

[CYP2J2]

other gene names :

[CYP2J2; CYP2J2; CPJ2]

uniprot entry name :

CP2J2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

502

specificity :

Recognizes endogenous levels of Cytochrome P450 2J2 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

app notes :

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info2 :

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 2J2. The exact sequence is proprietary.

products description :

Rabbit polyclonal antibody to Cytochrome P450 2J2

ncbi gi num :

18491008

ncbi acc num :

NP_000766.2

ncbi gb acc num :

NM_000775.2

uniprot acc num :

P51589

ncbi mol weight :

57,611 Da

ncbi pathways :

Arachidonate Epoxygenase / Epoxide Hydrolase Pathway (198838); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (105698); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Fatty Acids Pathway (105706); Inflammatory Mediator Regulation Of TRP Channels Pathway (948277); Inflammatory Mediator Regulation Of TRP Channels Pathway (948307); Linoleic Acid Metabolism Pathway (82992)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. [provided by RefSeq, Jul 2008]

uniprot summary :

CYP2J2: This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools. Belongs to the cytochrome P450 family. Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; EC 1.14.14.1; Contractile. Chromosomal Location of Human Ortholog: 1p31.3-p31.2. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm. Molecular Function: arachidonic acid 14,15-epoxygenase activity; iron ion binding; heme binding; arachidonic acid 11,12-epoxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding. Biological Process: xenobiotic metabolic process; exogenous drug catabolic process; icosanoid metabolic process; arachidonic acid metabolic process; linoleic acid metabolic process; epoxygenase P450 pathway; regulation of heart contraction

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage