Anti-TEAD1 Antibody | MyBioSource MBS821966 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-TEAD1 Antibody

catalog :

MBS821966

quantity :

0.03 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken, cow

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS821966

products type :

Antibody

products full name :

Anti-TEAD1 Antibody

products short name :

TEAD1

products name syn :

TCF13; TEF1; Transcriptional enhancer factor TEF-1; NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13

other names :

transcriptional enhancer factor TEF-1; Transcriptional enhancer factor TEF-1; transcriptional enhancer factor TEF-1; protein GT-IIC; transcription factor 13; transcriptional enhancer factor 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13

products gene name :

TEAD1

other gene names :

TEAD1; TEAD1; AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1; TCF13; TEF1; TEAD-1; TCF-13

uniprot entry name :

TEAD1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat, Bovine, Chicken, Dog, Pig

sequence length :

426

specificity :

Recognizes endogenous levels of TEAD1 protein.

purity :

The antibody was purified by immunogen affinity chromatography.

form :

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)

app notes :

WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500). Western blot analysis of TEAD1 expression in A431 (A), Raw264.7 (B), PC12 (C) whole cell lysates. Immunofluorescent analysis of TEAD1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

other info1 :

Immunogen: KLH-Conjugated synthetic peptide encompassing a sequence within the center region of human TEAD1. The exact seqence is proprietary.

ncbi gi num :

296434319

ncbi acc num :

NP_068780.2

ncbi gb acc num :

NM_021961.5

uniprot acc num :

P28347

ncbi pathways :

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544); YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway (576276)

ncbi summary :

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq, May 2010]

uniprot summary :

TEAD1: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC enhansons (5 -GTGGAATGT-3 ) and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif. Interacts with YAP1 and WWTR1/TAZ. Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm. Molecular Function: protein binding; DNA binding; transcription factor activity. Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression. Disease: Sveinsson Chorioretinal Atrophy

size1 :

0.03 mL

price1 :

155 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

295

more info or order :

MyBioSource product webpage