product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-MUC16 Antibody
catalog :
MBS8219219
quantity :
0.03 mL
price :
155 USD
clonality :
polyclonal
host :
unidentified
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of MUC16 expression in MCF7 (A), H9C2 (B), PC12 (C) whole cell lysates.
image 2 :
Immunohistochemical analysis of MUC16 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
image 3 :
Immunofluorescent analysis of MUC16 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
product information
catalog number :
MBS8219219
products type :
Antibody
products full name :
Anti-MUC16 Antibody
products short name :
[MUC16]
products name syn :
[CA125; Mucin-16; MUC-16; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125]
other names :
[mucin-16; Mucin-16; mucin-16; mucin 16, cell surface associated; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125]
products gene name :
[MUC16]
other gene names :
[MUC16; MUC16; CA125; CA125; MUC-16; CA-125]
uniprot entry name :
MUC16_HUMAN
clonality :
Polyclonal
host :
unknown
reactivity :
Human, Rat
sequence length :
14507
specificity :
Recognizes endogenous levels of MUC16 protein.
purity :
The antibody was purified by immunogen affinity chromatography.
form :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
storage stability :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
tested application :
WB, IH, IF/IC
app notes :
WB (1/500 - 1/1000). IH (1/100 - 1/200). IF/IC (1/100 - 1/500)
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Immunofluorescence (IF)
other info1 :
. Source: Rabbit. Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MUC16. The exact sequence is proprietary.
products description :
Rabbit polyclonal antibody to MUC16
ncbi gi num :
83367077
ncbi acc num :
NP_078966.2
ncbi gb acc num :
NM_024690.2
uniprot acc num :
Q8WXI7
ncbi pathways :
Metabolism Of Proteins Pathway (1268677); O-linked Glycosylation Pathway (1268734); O-linked Glycosylation Of Mucins Pathway (1268736); Post-translational Protein Modification Pathway (1268701); Termination Of O-glycan Biosynthesis Pathway (1268737)
uniprot summary :
MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Protein type: Membrane protein, peripheral; Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; plasma membrane; integral to membrane; vesicle. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification; cell adhesion
size1 :
0.03 mL
price1 :
155 USD
size2 :
0.1 mL
price2 :
220
size3 :
0.2 mL
price3 :
295
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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